Improvement of innate immune responses and defense activity in tiger shrimp (Penaeus monodon Fab.) by intramuscular administration of the Outer Membrane Protein Vibrio alginolyticus

E. Prasetio; A. Sudianto; M. Rozik; R. Nurdiyani; E. Sanusi; H. Nursyam; F. Fariedah; Marsoedi; Murachman

doi:10.1186/2193-1801-2-432

Improvement of innate immune responses and defense activity in tiger shrimp (Penaeus monodon Fab.) by intramuscular administration of the Outer Membrane Protein Vibrio alginolyticus

Maftuch^*
, E. Prasetio
, A. Sudianto
, M. Rozik
, R. Nurdiyani
, E. Sanusi
, H. Nursyam
, F. Fariedah
, Marsoedi
, Murachman

^*Corresponding author for this work

Faculty of Fisheries and Marine Sciences

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

The Outer Membrane Protein (OMP) of Vibrio alginolyticus cell wall was administered intramuscularly (IM) to the tiger shrimp (Penaeus monodon Fab.) at 10, 20, 30 μg/kg bw. After 14 days infection, the tiger shrimps were challenged with 107 bacterial density of Vibrio harveyi for 24 hours. The total haemocyte count (THC), differential haemocyte count (DHC) and amount of total protein plasma (TPP), superoxide dismutase and protease enzyme activity were monitored. The results showed that intramuscular administration of OMP enhanced an immunomodulatory effect and protection against V. harveyi. The beneficial effect of OMP on the tiger shrimp is dose-dependent and OMP-20 μg/kg bw is an optimal dose after two times of boosters for 14 days against V. harveyi infection.

Original language	English
Article number	432
Pages (from-to)	1-8
Number of pages	8
Journal	SpringerPlus
Volume	2
Issue number	1
DOIs	https://doi.org/10.1186/2193-1801-2-432
Publication status	Published - 2013

Keywords

Immunomodulation
Outer membrane protein
Tiger shrimp
Vibrio harveyi

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10.1186/2193-1801-2-432

Cite this

Maftuch, Prasetio, E., Sudianto, A., Rozik, M., Nurdiyani, R., Sanusi, E., Nursyam, H., Fariedah, F., Marsoedi, & Murachman (2013). Improvement of innate immune responses and defense activity in tiger shrimp (Penaeus monodon Fab.) by intramuscular administration of the Outer Membrane Protein Vibrio alginolyticus. SpringerPlus, 2(1), 1-8. Article 432. https://doi.org/10.1186/2193-1801-2-432

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title = "Improvement of innate immune responses and defense activity in tiger shrimp (Penaeus monodon Fab.) by intramuscular administration of the Outer Membrane Protein Vibrio alginolyticus",

abstract = "The Outer Membrane Protein (OMP) of Vibrio alginolyticus cell wall was administered intramuscularly (IM) to the tiger shrimp (Penaeus monodon Fab.) at 10, 20, 30 μg/kg bw. After 14 days infection, the tiger shrimps were challenged with 107 bacterial density of Vibrio harveyi for 24 hours. The total haemocyte count (THC), differential haemocyte count (DHC) and amount of total protein plasma (TPP), superoxide dismutase and protease enzyme activity were monitored. The results showed that intramuscular administration of OMP enhanced an immunomodulatory effect and protection against V. harveyi. The beneficial effect of OMP on the tiger shrimp is dose-dependent and OMP-20 μg/kg bw is an optimal dose after two times of boosters for 14 days against V. harveyi infection.",

keywords = "Immunomodulation, Outer membrane protein, Tiger shrimp, Vibrio harveyi",

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Maftuch, Prasetio, E, Sudianto, A, Rozik, M, Nurdiyani, R, Sanusi, E, Nursyam, H , Fariedah, F, Marsoedi & Murachman 2013, 'Improvement of innate immune responses and defense activity in tiger shrimp (Penaeus monodon Fab.) by intramuscular administration of the Outer Membrane Protein Vibrio alginolyticus', SpringerPlus, vol. 2, no. 1, 432, pp. 1-8. https://doi.org/10.1186/2193-1801-2-432

TY - JOUR

T1 - Improvement of innate immune responses and defense activity in tiger shrimp (Penaeus monodon Fab.) by intramuscular administration of the Outer Membrane Protein Vibrio alginolyticus

AU - Maftuch,

AU - Prasetio, E.

AU - Sudianto, A.

AU - Rozik, M.

AU - Nurdiyani, R.

AU - Sanusi, E.

AU - Nursyam, H.

AU - Fariedah, F.

AU - Marsoedi,

AU - Murachman,

PY - 2013

Y1 - 2013

N2 - The Outer Membrane Protein (OMP) of Vibrio alginolyticus cell wall was administered intramuscularly (IM) to the tiger shrimp (Penaeus monodon Fab.) at 10, 20, 30 μg/kg bw. After 14 days infection, the tiger shrimps were challenged with 107 bacterial density of Vibrio harveyi for 24 hours. The total haemocyte count (THC), differential haemocyte count (DHC) and amount of total protein plasma (TPP), superoxide dismutase and protease enzyme activity were monitored. The results showed that intramuscular administration of OMP enhanced an immunomodulatory effect and protection against V. harveyi. The beneficial effect of OMP on the tiger shrimp is dose-dependent and OMP-20 μg/kg bw is an optimal dose after two times of boosters for 14 days against V. harveyi infection.

AB - The Outer Membrane Protein (OMP) of Vibrio alginolyticus cell wall was administered intramuscularly (IM) to the tiger shrimp (Penaeus monodon Fab.) at 10, 20, 30 μg/kg bw. After 14 days infection, the tiger shrimps were challenged with 107 bacterial density of Vibrio harveyi for 24 hours. The total haemocyte count (THC), differential haemocyte count (DHC) and amount of total protein plasma (TPP), superoxide dismutase and protease enzyme activity were monitored. The results showed that intramuscular administration of OMP enhanced an immunomodulatory effect and protection against V. harveyi. The beneficial effect of OMP on the tiger shrimp is dose-dependent and OMP-20 μg/kg bw is an optimal dose after two times of boosters for 14 days against V. harveyi infection.

KW - Immunomodulation

KW - Outer membrane protein

KW - Tiger shrimp

KW - Vibrio harveyi

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ER -

Improvement of innate immune responses and defense activity in tiger shrimp (Penaeus monodon Fab.) by intramuscular administration of the Outer Membrane Protein Vibrio alginolyticus

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Keywords

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