Identification of Genetic Diversity of Bone Morphogenetic Protein (Bmp15) Genes in Senduro Goats and Boerawa Goats using Pcr-Sequencing

The first step to produce Marker Assisted Selection (MAS) in Senduro goats and Boerawa goats is to identify candidate genes related to litter size traits, one of which is the Bone Morphogenetic Protein (BMP15) gene. The study aimed to identify the genetic diversity of BMP15 exon 2 genes in Senduro goats and Boerawa goats in UPT PT-HMT Singosari, using PCR-Sequencing. This study used a survey method, sampling by means of purposive sampling, blood samples of Senduro and Boerawa goats were collected from UPT PT-HMT Singosari. The samples used were 27 samples of Senduro goats and 20 samples of Boerawa goats. DNA amplification was carried out at the Biotechnology Laboratory, Faculty of Animal Science, Brawijaya University, Malang. For the sequencing process, the PCr product were sent to 1^st Base Laboratory (Genetica Science), Selangor Malaysia.The sequencing method used is the Sanger method, using the ABI Prims 3100-Avant Genetic Analyzer sequencer machine. Alignment the result of the sequencing in the form of chromatograms were analyzed using BioEdit and Mega X software. The association of genetic diversity of the BMP15 gene with litter size was carried out by T-test analysis. The sequencing results showed that the Boerawa goat sample (BC 11) experienced a mutation, there was a change in the base from Cytosine (C) to Guanine (G) at the position of 709 bp (C709G). In the Boerawa goat sample (BC 18) also mutated, there was a change in the base from Cytosine (C) to Guanine (G) at the position of 707 bp (C707G). No mutations were found in the Senduro goat samples because there was no change in the base for all Senduro goat samples. All samples of Senduro goats carry the base Cytosine (C). The results showed that the identification of the BMP15 exon 2 gene with Forward 5’-GACCCTTCTTCTCTTG-3’ and Reverse 5’-CATCTCTGCTCCATACAC-3’ primers along 990 bp in Senduro goats using PCR-sequencing was uniform (monomorphic). Identifying the BMP15-2 exon 2 gene for Boerawa goats is polymorphic, but cannot be associated with litter size.