Identification indigenous Yeast from Palm Juice Cocos nucifera L for Bioethanol Production

The search for alternative and renewable fuel sources has become a major concern all over the world because of its environmental friendliness and environmental sustainability. Sources of raw materials include bioethanol. Bioethanol as raw material to replace fossil fuels because fossil fuels cause global warming and the greenhouse effect. High carbon source material is a source of bioethanol that renewable, effective, and abundant. Bioethanol from palm juice can be a promising technology because of its high sugar content. The goals of this research is to screen and identify indegenous yeast from palm juice Cocos nucifera L which is potential for bioethanol production. Isolation of yeast from palm juice C.nucifera L using YMEA medium. Purification used coconut water media. Screening used several parameters: bioethanol content (using alcohol meter), reducing sugar content (DNS method), and cells number (spectrophotometer 600 nm). Identification used 18S DNA. Five yeast isolates were produced, namely K3D, K21A, K1C1, K2C, and K1A. The superior isolates was K1A (16%). The isolate was similar with Pichia manshurica strain IFO 10726 with 99.99 similarty value.