Hepatoprotective Effect of Tocotrienol Rich Fraction From palm fatty acid distillate on liver peroxidation induced by thermally oxidized frying oil

Tocotrienol rich fraction (TRF) from palm fatty acid distillate (PFAD) was examined its ability to protect liver from lipid peroxidation induced by thermally oxidized frying oil. Six groups of rats were treated by TRF 0, 25, 50, and 75 mg/kg bw/day and commercial vitamin E at dose of 75 mg/kg bw/day. The normal rats was used as a control group. Hepatoprotective effect of TRF was measured by liver malondialdehyde (MDA) level, superoxide dismutase activity, catalase activity, and hepar histopathology. The results showed that TRF from PFAD was effective antioxidant to protect liver from peroxidation, indicated by decreasing liver MDA level, increasing liver SOD activity, and increasing liver catalase activity, as well as impairment in hepatocytes after TRF force feeding. The effective dose of TRF was 50 mg/kg bw/day. At higher dose (75 mg/kg bw/day) TRF caused highest average number of hepatocellular degeneration and revealed more pronounce oxidative damage than TRF dose of 50 mg/kg bw/day. At lower dose (25 mg/kg bw/day) TRF was not effective to prevent liver from lipid peroxidation. TRF was more effective as hepatoprotector than commercial vitamin E.