Genetic differences and population structure of spotted barb (cyprinidae) collected from three rivers in Java Island

Barbodes binotatus is an endemic species and most dominant freshwater fish in Indonesia. This prelilinary study aimed to calculate genetic differences of the species from three different rivers in Java island. A total of 90 specimens were collected from each river and a total of 26 sequences were analyzed using mitochondrial DNA (mtDNA) region Cytochrome Oxidase Subunit I (COI). BLAST analysis showed > 97 % similarity of B. binotatus with another research. The result of sequence analysis showed that B. binotatus from Central Java were not significantly different from B. binotatus from East Java, and B. binotatus from West Java were significantly different from two others. The genetic differences of B. binotatus from three locations were observed in five basepair (335 bp, 399 bp,449 bp, 497 bp, and 545 bp). The result of population structure analysis showed an FST value of 1.00. It suggested that there was genetic differentiation between populations of Pasuruan, Yogyakarta and Cirebon. Inter-location analysis requires to be done to confirm the genetic differences from another regions.