Feed improvement and laserpuncture induction to improve the gynogenetic quality of catfish (Clarias gariepinus) fry

An improved feed and laserpuncture induction have been demonstrated to accelerate the gonadal maturation of reproductive catfish (Clarias gariepinus) However, it is relatively unclear whether broodstock semen, when irradiated with ultraviolet (UV), will produce the same results in egg fertilization by gynogenesis techniques. Gynogenesis is a parthenogenesis reproduction technique where embryonic development occurs by obtaining genetic material only from females, without male contribution. This study aimed to determine whether the distance of UV radiation on catfish (Clarias gariepinus) broodstock semen enhanced by feed improvement and laserpuncture induction will successfully fertilize eggs. The distance between the UV source and semen was divided into 4 treatments namely: control, distance of 10 cm, 15 cm, and 20 cm, with 6 replications. Furthermore, the data collection technique involved a completely randomized design (CRD). The parameters observed included the Hatching Rate (HR), Survival Rate (SR), and Specific Growth Rate Length (SGRL) of the larvae, until the fry were 6 weeks old. The results showed that 20 cm UV radiation distance on semen had a significant effect (p<0.05) on the increasing percentage of HR values (63%), SR values (54%), and SGRL (83%) compared to other treatments.