Exploring of Cheral® potential as anticancer agent via apoptosis mechanism

Sapti Puspitarini; Noviana Dwi Lestari; Muhaimin Rifai

doi:10.1063/1.5125553

Exploring of Cheral^® potential as anticancer agent via apoptosis mechanism

Sapti Puspitarini
, Widodo
, Noviana Dwi Lestari
, Muhaimin Rifai^*

^*Corresponding author for this work

Faculty of Mathematics and Natural Sciences

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Citations (Scopus)

Abstract

Cancer is characterized by cell growth uncontrolled in tissue and doesn't recognize apoptosis. Cheral^® is an herbal medicinal product that contains meniran (Phyllanthus niruri) and turmeric (Curcuma longa). Those herbals have been known to contain some active compounds which have the pharmacological function, such as anticancer. Therefore, this study aims to identify Cheral® potential in inducing apoptosis in T47D breast cancer cells line as well as to describe their mechanism to induce apoptosis by in vitro and bioinformatic analysis. For the in vitro study, AnnexinV/PI dye was used and then analyzed using flow cytometry for apoptosis assay. Bioinformatic analysis was conducted for exploring mechanism Cheral^® to induce apoptosis. The result from apoptosis assay showed that the Cheral^® could induce apoptosis in T47D cells. Further investigation by bioinformatics indicated that some active compounds in Cheral^® helped to regulate several proteins that involve in the apoptosis process. It is concluded that the Cheral^® could have the potential as an anticancer in T47D cells through their ability to induce apoptosis. Further research regarding proteins related to such apoptosis process may prove necessary.

Original language	English
Title of host publication	Proceedings of the 2nd International Conference on Biosciences and Medical Engineering, ICBME 2019
Subtitle of host publication	Towards Innovative Research and Cross-Disciplinary Collaborations
Editors	Naji Arafat Mahat, Roswanira Ab. Wahab, Fahrul Zaman Huyop, Aemi Syazwani Abdul Keyon, Nursyafreena Binti Attan, Sheela Chandren, Ida Bagus Wayan Gunam
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735419001
DOIs	https://doi.org/10.1063/1.5125553
Publication status	Published - 6 Sept 2019
Event	2nd International Conference on Biosciences and Medical Engineering 2019: Towards Innovative Research and Cross-Disciplinary Collaborations, ICBME 2019 - Bali, Indonesia Duration: 11 Apr 2019 → 12 Apr 2019

Publication series

Name	AIP Conference Proceedings
Volume	2155
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	2nd International Conference on Biosciences and Medical Engineering 2019: Towards Innovative Research and Cross-Disciplinary Collaborations, ICBME 2019
Country/Territory	Indonesia
City	Bali
Period	11/04/19 → 12/04/19

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Access to Document

10.1063/1.5125553

Cite this

Puspitarini, S., Widodo, Lestari, N. D., & Rifai, M. (2019). Exploring of Cheral^® potential as anticancer agent via apoptosis mechanism. In N. A. Mahat, R. A. Wahab, F. Z. Huyop, A. S. A. Keyon, N. B. Attan, S. Chandren, & I. B. W. Gunam (Eds.), Proceedings of the 2nd International Conference on Biosciences and Medical Engineering, ICBME 2019: Towards Innovative Research and Cross-Disciplinary Collaborations Article 020049 (AIP Conference Proceedings; Vol. 2155). American Institute of Physics Inc.. https://doi.org/10.1063/1.5125553

Puspitarini, Sapti ; Widodo ; Lestari, Noviana Dwi et al. / Exploring of Cheral^® potential as anticancer agent via apoptosis mechanism. Proceedings of the 2nd International Conference on Biosciences and Medical Engineering, ICBME 2019: Towards Innovative Research and Cross-Disciplinary Collaborations. editor / Naji Arafat Mahat ; Roswanira Ab. Wahab ; Fahrul Zaman Huyop ; Aemi Syazwani Abdul Keyon ; Nursyafreena Binti Attan ; Sheela Chandren ; Ida Bagus Wayan Gunam. American Institute of Physics Inc., 2019. (AIP Conference Proceedings).

@inproceedings{fe04c9c8c23a4ffaaaa0acd5b915a5a2,

title = "Exploring of Cheral{\textregistered} potential as anticancer agent via apoptosis mechanism",

abstract = "Cancer is characterized by cell growth uncontrolled in tissue and doesn't recognize apoptosis. Cheral{\textregistered} is an herbal medicinal product that contains meniran (Phyllanthus niruri) and turmeric (Curcuma longa). Those herbals have been known to contain some active compounds which have the pharmacological function, such as anticancer. Therefore, this study aims to identify Cheral{\textregistered} potential in inducing apoptosis in T47D breast cancer cells line as well as to describe their mechanism to induce apoptosis by in vitro and bioinformatic analysis. For the in vitro study, AnnexinV/PI dye was used and then analyzed using flow cytometry for apoptosis assay. Bioinformatic analysis was conducted for exploring mechanism Cheral{\textregistered} to induce apoptosis. The result from apoptosis assay showed that the Cheral{\textregistered} could induce apoptosis in T47D cells. Further investigation by bioinformatics indicated that some active compounds in Cheral{\textregistered} helped to regulate several proteins that involve in the apoptosis process. It is concluded that the Cheral{\textregistered} could have the potential as an anticancer in T47D cells through their ability to induce apoptosis. Further research regarding proteins related to such apoptosis process may prove necessary.",

author = "Sapti Puspitarini and Widodo and Lestari, \{Noviana Dwi\} and Muhaimin Rifai",

note = "Publisher Copyright: {\textcopyright} 2019 Author(s).; 2nd International Conference on Biosciences and Medical Engineering 2019: Towards Innovative Research and Cross-Disciplinary Collaborations, ICBME 2019 ; Conference date: 11-04-2019 Through 12-04-2019",

year = "2019",

month = sep,

day = "6",

doi = "10.1063/1.5125553",

language = "English",

series = "AIP Conference Proceedings",

publisher = "American Institute of Physics Inc.",

editor = "Mahat, \{Naji Arafat\} and Wahab, \{Roswanira Ab.\} and Huyop, \{Fahrul Zaman\} and Keyon, \{Aemi Syazwani Abdul\} and Attan, \{Nursyafreena Binti\} and Sheela Chandren and Gunam, \{Ida Bagus Wayan\}",

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Puspitarini, S, Widodo, Lestari, ND & Rifai, M 2019, Exploring of Cheral^® potential as anticancer agent via apoptosis mechanism. in NA Mahat, RA Wahab, FZ Huyop, ASA Keyon, NB Attan, S Chandren & IBW Gunam (eds), Proceedings of the 2nd International Conference on Biosciences and Medical Engineering, ICBME 2019: Towards Innovative Research and Cross-Disciplinary Collaborations., 020049, AIP Conference Proceedings, vol. 2155, American Institute of Physics Inc., 2nd International Conference on Biosciences and Medical Engineering 2019: Towards Innovative Research and Cross-Disciplinary Collaborations, ICBME 2019, Bali, Indonesia, 11/04/19. https://doi.org/10.1063/1.5125553

Exploring of Cheral^® potential as anticancer agent via apoptosis mechanism. / Puspitarini, Sapti; Widodo; Lestari, Noviana Dwi et al.
Proceedings of the 2nd International Conference on Biosciences and Medical Engineering, ICBME 2019: Towards Innovative Research and Cross-Disciplinary Collaborations. ed. / Naji Arafat Mahat; Roswanira Ab. Wahab; Fahrul Zaman Huyop; Aemi Syazwani Abdul Keyon; Nursyafreena Binti Attan; Sheela Chandren; Ida Bagus Wayan Gunam. American Institute of Physics Inc., 2019. 020049 (AIP Conference Proceedings; Vol. 2155).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Exploring of Cheral® potential as anticancer agent via apoptosis mechanism

AU - Puspitarini, Sapti

AU - Widodo,

AU - Lestari, Noviana Dwi

AU - Rifai, Muhaimin

PY - 2019/9/6

Y1 - 2019/9/6

N2 - Cancer is characterized by cell growth uncontrolled in tissue and doesn't recognize apoptosis. Cheral® is an herbal medicinal product that contains meniran (Phyllanthus niruri) and turmeric (Curcuma longa). Those herbals have been known to contain some active compounds which have the pharmacological function, such as anticancer. Therefore, this study aims to identify Cheral® potential in inducing apoptosis in T47D breast cancer cells line as well as to describe their mechanism to induce apoptosis by in vitro and bioinformatic analysis. For the in vitro study, AnnexinV/PI dye was used and then analyzed using flow cytometry for apoptosis assay. Bioinformatic analysis was conducted for exploring mechanism Cheral® to induce apoptosis. The result from apoptosis assay showed that the Cheral® could induce apoptosis in T47D cells. Further investigation by bioinformatics indicated that some active compounds in Cheral® helped to regulate several proteins that involve in the apoptosis process. It is concluded that the Cheral® could have the potential as an anticancer in T47D cells through their ability to induce apoptosis. Further research regarding proteins related to such apoptosis process may prove necessary.

AB - Cancer is characterized by cell growth uncontrolled in tissue and doesn't recognize apoptosis. Cheral® is an herbal medicinal product that contains meniran (Phyllanthus niruri) and turmeric (Curcuma longa). Those herbals have been known to contain some active compounds which have the pharmacological function, such as anticancer. Therefore, this study aims to identify Cheral® potential in inducing apoptosis in T47D breast cancer cells line as well as to describe their mechanism to induce apoptosis by in vitro and bioinformatic analysis. For the in vitro study, AnnexinV/PI dye was used and then analyzed using flow cytometry for apoptosis assay. Bioinformatic analysis was conducted for exploring mechanism Cheral® to induce apoptosis. The result from apoptosis assay showed that the Cheral® could induce apoptosis in T47D cells. Further investigation by bioinformatics indicated that some active compounds in Cheral® helped to regulate several proteins that involve in the apoptosis process. It is concluded that the Cheral® could have the potential as an anticancer in T47D cells through their ability to induce apoptosis. Further research regarding proteins related to such apoptosis process may prove necessary.

UR - https://www.scopus.com/pages/publications/85072603083

U2 - 10.1063/1.5125553

DO - 10.1063/1.5125553

M3 - Conference contribution

AN - SCOPUS:85072603083

T3 - AIP Conference Proceedings

BT - Proceedings of the 2nd International Conference on Biosciences and Medical Engineering, ICBME 2019

A2 - Mahat, Naji Arafat

A2 - Wahab, Roswanira Ab.

A2 - Huyop, Fahrul Zaman

A2 - Keyon, Aemi Syazwani Abdul

A2 - Attan, Nursyafreena Binti

A2 - Chandren, Sheela

A2 - Gunam, Ida Bagus Wayan

PB - American Institute of Physics Inc.

T2 - 2nd International Conference on Biosciences and Medical Engineering 2019: Towards Innovative Research and Cross-Disciplinary Collaborations, ICBME 2019

Y2 - 11 April 2019 through 12 April 2019

ER -

Puspitarini S, Widodo, Lestari ND, Rifai M. Exploring of Cheral^® potential as anticancer agent via apoptosis mechanism. In Mahat NA, Wahab RA, Huyop FZ, Keyon ASA, Attan NB, Chandren S, Gunam IBW, editors, Proceedings of the 2nd International Conference on Biosciences and Medical Engineering, ICBME 2019: Towards Innovative Research and Cross-Disciplinary Collaborations. American Institute of Physics Inc. 2019. 020049. (AIP Conference Proceedings). doi: 10.1063/1.5125553