TY - GEN
T1 - Ethanolic extract of centella asiatica increased IGF-1 and IRS expression in zebrafish (Danio rerio) rotenone-induced
AU - Cory'ah, Fitra Arsy Nur
AU - Primaditya, Vanda
AU - Ariati, Linda Ika Puspita
AU - Zakiah,
AU - Wardani, Dyah Woro Kartiko Kusumo
AU - Primihastuti, Dianita
AU - Yuningsih,
AU - Khotimah, Husnul
AU - Ali, Mohammad Muljohadi
AU - Nurdiana,
N1 - Publisher Copyright:
© 2020 American Institute of Physics Inc.. All rights reserved.
PY - 2020/4/21
Y1 - 2020/4/21
N2 - IRS (insulin receptor substrate) is responsible for signal transduction of Insulin-like growth factor-1 (IGF-1) for cell differentiation, proliferation, and anti-apoptosis. Centella asiatica (CA) contains triterpenes as antioxidant and anti-inflammatory, macro and micronutrients. This study observed the effect of CA extract on the expression of IGF-1, IRS, and the linear growth of rotenone-induced zebrafish larvae. The zebrafish embryos were divided into five groups: control, Rotenone 12 ppb, Rotenone, and CA extract concentration 2.5, 5, and 10 μg/ml, respectively exposed from 2-hours post fertilization (hpf) until 3-days post-fertilization (dpf). The expression of IGF-1 and IRS were conducted at 9 dpf by immunehistochemistry whole-mount. The body length was measured at 3, 6, and 9 dpf using Image Raster software. The data were analyzed using one-way ANOVA. The results showed that the administration of CA extract could increase the expression of IGF-1 and IRS in rotenone-induced zebrafish larvae. The measurement of body length showed that rotenone reduced the body length of larvae after 6 dpf, and 5 μg/mL of CA extract significantly increased the body length at 6 and 9 dpf. It can be concluded that CA extract increased the body length of zebrafish larvae through the increasing of IGF-1 and IRS signaling.
AB - IRS (insulin receptor substrate) is responsible for signal transduction of Insulin-like growth factor-1 (IGF-1) for cell differentiation, proliferation, and anti-apoptosis. Centella asiatica (CA) contains triterpenes as antioxidant and anti-inflammatory, macro and micronutrients. This study observed the effect of CA extract on the expression of IGF-1, IRS, and the linear growth of rotenone-induced zebrafish larvae. The zebrafish embryos were divided into five groups: control, Rotenone 12 ppb, Rotenone, and CA extract concentration 2.5, 5, and 10 μg/ml, respectively exposed from 2-hours post fertilization (hpf) until 3-days post-fertilization (dpf). The expression of IGF-1 and IRS were conducted at 9 dpf by immunehistochemistry whole-mount. The body length was measured at 3, 6, and 9 dpf using Image Raster software. The data were analyzed using one-way ANOVA. The results showed that the administration of CA extract could increase the expression of IGF-1 and IRS in rotenone-induced zebrafish larvae. The measurement of body length showed that rotenone reduced the body length of larvae after 6 dpf, and 5 μg/mL of CA extract significantly increased the body length at 6 and 9 dpf. It can be concluded that CA extract increased the body length of zebrafish larvae through the increasing of IGF-1 and IRS signaling.
UR - https://www.scopus.com/pages/publications/85092600391
U2 - 10.1063/5.0002606
DO - 10.1063/5.0002606
M3 - Conference contribution
AN - SCOPUS:85092600391
T3 - AIP Conference Proceedings
BT - International Conference on Life Science and Technology, ICoLiST 2019
A2 - Taufiq, Ahmad
A2 - Susanto, Hendra
A2 - Nur, Hadi
A2 - Aziz, Muhammad
A2 - Chang, Chuang-Rung
A2 - Lee, Hyohyemi
A2 - Diantoro, Markus
A2 - Mufti, Nandang
A2 - Malek, Nik Ahmad Nizam Nik
A2 - Wang, I. Ching
A2 - Iskandar, Djoko Tjahnono
A2 - Elbers, Gereon
A2 - Sunaryono, Sunaryono
A2 - Zubaidah, Siti
A2 - Sumari, Sumari
A2 - Aulanni'am, null
A2 - Nandiyanto, Asep Bayu
A2 - Wibowo, Indra
A2 - Handaya, Adeodatus Yuda
PB - American Institute of Physics Inc.
T2 - 2nd International Conference on Life Science and Technology, ICoLiST 2019
Y2 - 12 September 2019 through 13 September 2019
ER -