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EMULSIFYING PROPERTIES AND ANTIOXIDANT ACTIVITY OF PHOSPHOLIPIDS FRACTIONS FROM PALM PRESSED FIBRE

  • Kgs Ahmadi*
  • , Teti Estiasih
  • , Ahmad Diaul Khuluq
  • *Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Palm pressed fibre (PPF) is a by-product of crude palm oil (CPO) extraction and has great potential as a source of phospholipids (PL). PPF was extracted from chloroform:methanol to extract total lipid, and polar lipid was separated using methanol. PL from PPF was fractionated by ethanol, and subsequently, the ethanol-soluble fraction was fractionated by acetone. Four fractions were obtained: ethanol-soluble, ethanol-insoluble, ethanol-acetone soluble, ethanol soluble-acetone insoluble. All fractions had different PL molecular species compositions. Different compositions of PL in the fractions resulted in different hydrophilic-lipophilic balance (HLB) values, emulsifying properties, and antioxidant activity. HLB value of crude PL from PPF was 6.07 that was suitable for water in oil (w/o) emulsion indicated by low emulsifying activity index (EAI) and emulsion stability index (ESI) in oil-water (o/w) emulsion systems. Ethanol-soluble and ethanol-acetone soluble fractions exhibited better emulsifying properties and higher HLB values of around 10. Crude PL and its fractions showed a potential antioxidant activity to prevent bulk soybean oil in a single antioxidant system. Synergism with α-tocopherol appeared in a binary antioxidant system indicated by low peroxide value compared to the single one. PL composition affected the ability of PL fractions as antioxidants.

Original languageEnglish
Pages (from-to)686-698
Number of pages13
JournalJournal of Oil Palm Research
Volume34
Issue number4
DOIs
Publication statusPublished - Dec 2022

Keywords

  • binary antioxidant system
  • emulsifying properties
  • fractionation
  • HLB value
  • palm pressed fibre

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