Effectiveness of Nanoparticle-based Young Palmyra Fruit Water–egg Yolk in Liquid Semen Diluent of Sumba Ongole Bulls

This research aims to determine the quality of liquid semen of Sumba Ongole (SO) bulls during cold storage in palmyra fruit water and egg yolk-based nanoparticle diluent. The research was conducted at the Animal Husbandry Service Laboratory of East Sumba Regency. Four SO Bulls heads aged between 3 and 4 years, weighing 272 to 525kg, were used in the study. They were maintained to meet healthy body conditions and normal reproduction. Semen was collected twice a week using an artificial vagina. This research method was through an experiment in the laboratory, which consisted of four treatments and 10 replications (semen collection). The treatments are T0: CEP-3 diluent (control); T1: 90% of young palmyra fruit water (PFW)+10% of egg yolk (EY); T2: 85% PFW+15% EY; T3: 80% PFW+20% EY; T4: 75% PFW+25% EY. The PFW diluent was filtered three times using membrane filters to obtain an average particle size of 580±35 nm. The measured variables were individual motility, concentration, total motile sperm, viability, abnormalities, intact plasma membrane, and acrosomal cap. The research used a randomized block design. Duncan's Multiple Range test was carried out to determine the difference between treatments. The results showed that the nanoparticle diluent from egg yolk and palmyra fruit water effectively maintained the quality and integrity of SO Bull's spermatozoa membranes. In conclusion, nanoparticle-based palmyra fruit water and egg yolk diluent effectively maintain the semen quality of Sumba Ongole Bulls.