Effect of the combined application of Lampung Robusta Coffee Extract and Lactobacillus acidophilus on the Ileum and Caecum Histopathology in Salmonella enterica infected Balb/C Mice

Salmonella enterica is a gram-negative bacterium that can cause Salmonellosis and gastroenteritis in humans and animals. Further, this bacterial infection is also associated with the reactive oxygen species (ROS) by lipid peroxidase that can destroy the intestinal cell's membrane. This study aimed to evaluate the preventive effect of the combined application of Lampung Robusta coffee extract and Lactobacillus acidophilus on the Ileum and Caecum Histopathology in Salmonella enterica infected Mice. In this study, male Balb-c mice aged between 8-10 weeks and weight 20-25 grams were used, these experimental animals were divided into six experimental groups namely K^- (Negative control without any infection), K⁺ (Positive control with S. enterica), KL (Only L. acidophilus treated mice), P1, P2, and P3 were given a preventive extract of coffee with a concentration of 250 mg/kg BW, 500 mg/kg BW, and 750 mg/kg BW respectively and L. acidophilus to S.enterica infected mice and arrange in completely Randomized Design. Descriptive histopathological analyses were carried out after HE staining and villi's length and width for ileum's histopathology and counting goblet cells for caecum's histopathology was scored. The results of the study revealed that administration of Robusta Coffee extract @ 250 mg/ kg BW and L. acidophilus has a preventive effect on the ileum and caecum damage caused by salmonellosis.