Effect of robusta coffee from lampung (Coffea canephora) to relative number of Ho-1, Nrf2 and duodenum tissue histopathology in chicken

Coffee contains high phenols, especially chlorogenic acid which acts as a modulation of the immune response, and antioxidants. Robusta coffee (Coffea canephora) is a type of coffee with high levels of chlorogenic acid and high caffeine content which has the potential as an antioxidant so that it can be used as a green coffee extract (GCE). Green coffee extract is made from roasted coffee beans. This study used 48 layer chickens, consisting of 4 treatments and 12 replications consisting of negative controls (healthy chickens without coffee supplementation), T1 (coffee extract dose 500 mg/kg BW), T2 (coffee extract dose 1000 mg/kg BW) and T3 (coffee extract dose 1500 mg/kg body weight. Data in the form of quantitative duodenum organ histopathology using haematoxylin and eosin staining and histopathological picture of duodenum were observed using a microscope and analyzed descriptively, test for Nrf2 and HO-1 with flow cytometri the result of robusta coffee (Coffea canephora) extract from lampung had a good effect at a dose of 500 mg/kg body weight against the relative number of Nrf2, HO-1, and histopathological improvement in the duodenum. The conclusion of this study shows that robusta coffe e (Coffea canephora) extract has the potential as an anti-inflammatory and antioxidant in chickens.