TY - JOUR
T1 - Effect of growth temperature, time, and storage on plasmid stability in recombinant Lactococcus lactis expressing SARS-CoV-2 HCR spike antigen
AU - Yurina, Valentina
AU - Freddy, Juan
AU - Wicaksono, Samuel Aryo
AU - Adianingsih, Oktavia Rahayu
AU - Triastuti, Efta
AU - Shimosato, Takeshi
N1 - Publisher Copyright:
Copyright Yurina V et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. https://creativecommons.org/licenses/by/4.0/
PY - 2026
Y1 - 2026
N2 - Plasmids serve as antigen vector carriers in live bacterial vaccines but face stability issues that can undermine their effectiveness. This study aimed to evaluate the effect of temperature and incubation time on the stability of a plasmid-based bacterial vaccine expressing the SARS-CoV-2 spike protein antigen. Plasmid stability was assessed based on the number of colonies formed under various incubation conditions, including temperature and incubation time. L. lactis plasmids were incubated in Elliker medium at temperatures of 25 °C, 30 °C, and 40 °C for 72 h, with evaluations at 24, 48, and 72 h. Results indicated that L. lactis at 30 °C yielded the most colonies, while 25 °C better preserved cell stability (p < 0.05). Protein stability was found to be higher when the cell pellets were stored at 4 °C compared to -20 °C, -40 °C, and -80 °C. In conclusion, bacterial growth conditions influence plasmid stability, while storage conditions impact protein expression.
AB - Plasmids serve as antigen vector carriers in live bacterial vaccines but face stability issues that can undermine their effectiveness. This study aimed to evaluate the effect of temperature and incubation time on the stability of a plasmid-based bacterial vaccine expressing the SARS-CoV-2 spike protein antigen. Plasmid stability was assessed based on the number of colonies formed under various incubation conditions, including temperature and incubation time. L. lactis plasmids were incubated in Elliker medium at temperatures of 25 °C, 30 °C, and 40 °C for 72 h, with evaluations at 24, 48, and 72 h. Results indicated that L. lactis at 30 °C yielded the most colonies, while 25 °C better preserved cell stability (p < 0.05). Protein stability was found to be higher when the cell pellets were stored at 4 °C compared to -20 °C, -40 °C, and -80 °C. In conclusion, bacterial growth conditions influence plasmid stability, while storage conditions impact protein expression.
KW - Plasmid
KW - protein expression
KW - stability
KW - storage
KW - temperature
UR - https://www.scopus.com/pages/publications/105045034720
U2 - 10.3897/pharmacia.73.e185770
DO - 10.3897/pharmacia.73.e185770
M3 - Article
AN - SCOPUS:105045034720
SN - 0428-0296
VL - 73
JO - Pharmacia
JF - Pharmacia
M1 - e185770
ER -