Effect of centella asiatica to the glucose transporter 4 and osteocalcin on the rotenonee-induced zebrafish larvae (Danio rerio) stunting model

Vanda Primaditya; Fitra Arsy Nur Cory'ah; Linda Ika Puspita Ariati; Zakiah; Dyah Woro Kartiko Kusumo Wardani; Yuningsih; Dianita Primihastuti; Husnul Khotimah; Mohammad Muljohadi Ali; Wibi Riawan

doi:10.1063/5.0002607

Effect of centella asiatica to the glucose transporter 4 and osteocalcin on the rotenonee-induced zebrafish larvae (Danio rerio) stunting model

Vanda Primaditya
, Fitra Arsy Nur Cory'ah
, Linda Ika Puspita Ariati
, Zakiah
, Dyah Woro Kartiko Kusumo Wardani
, Yuningsih
, Dianita Primihastuti
, Husnul Khotimah^*
, Mohammad Muljohadi Ali
, Wibi Riawan

^*Corresponding author for this work

Faculty of Medicine

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

4 Citations (Scopus)

Abstract

Stunting is a failure of linear growth during the first two years of life. Rotenone is pesticide causes stunting. Rotenone as Endocrine Disrupting Chemicals and complex I mitochondrial inhibitor thus causes a decrease of ATP and oxidative stress. Centella asiatica is a plant that contains high antioxidants and antiinflammation. The purpose of this study is to evaluate the effect of CA ethanolic extract toward the rotenone-induced zebrafish larvae stunting model toward the expression increasing of GLUT 4 and Osteocalcin. This experimental study used zebrafish larvae as an animal model. Rotenone 12 ppb and CA extract were administered at 2-72 hpf. Observation of body length using optilab Image Raster at 3, 6, and 9 dpf, while the GLUT 4 and osteocalcin observed at 9 dpf by wholemount immunostaining. The results showed that rotenone 12.5 ppb decreased the body length of zebrafish larvae at 6 and 9 dpf significantly and more than 2 SD shorter from control. The expression of GLUT 4 and Osteocalcin in zebrafish larvae in the rotenone-induced group decreased significantly. Administration of 5 μg/mL extract increased expression of GLUT4, while the expression of osteocalcin increased by 2.5 μg/mL and 5 μg/mL. Rotenone 12.5 ppb induced stunting of zebrafish larvae. Ethanolic extract of CA protects the zebrafish larvae from stunting through increased expression of GLUT 4 and Osteocalcin.

Original language	English
Title of host publication	International Conference on Life Science and Technology, ICoLiST 2019
Editors	Ahmad Taufiq, Hendra Susanto, Hadi Nur, Muhammad Aziz, Chuang-Rung Chang, Hyohyemi Lee, Markus Diantoro, Nandang Mufti, Nik Ahmad Nizam Nik Malek, I. Ching Wang, Djoko Tjahnono Iskandar, Gereon Elbers, Sunaryono Sunaryono, Siti Zubaidah, Sumari Sumari, Aulanni'am, Asep Bayu Nandiyanto, Indra Wibowo, Adeodatus Yuda Handaya
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735419902
DOIs	https://doi.org/10.1063/5.0002607
Publication status	Published - 21 Apr 2020
Event	2nd International Conference on Life Science and Technology, ICoLiST 2019 - Malang, East Java, Indonesia Duration: 12 Sept 2019 → 13 Sept 2019

Publication series

Name	AIP Conference Proceedings
Volume	2231
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	2nd International Conference on Life Science and Technology, ICoLiST 2019
Country/Territory	Indonesia
City	Malang, East Java
Period	12/09/19 → 13/09/19

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 2 Zero Hunger

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10.1063/5.0002607

Cite this

Primaditya, V., Cory'ah, F. A. N., Ariati, L. I. P., Zakiah, Wardani, D. W. K. K., Yuningsih, Primihastuti, D., Khotimah, H., Ali, M. M., & Riawan, W. (2020). Effect of centella asiatica to the glucose transporter 4 and osteocalcin on the rotenonee-induced zebrafish larvae (Danio rerio) stunting model. In A. Taufiq, H. Susanto, H. Nur, M. Aziz, C.-R. Chang, H. Lee, M. Diantoro, N. Mufti, N. A. N. N. Malek, I. C. Wang, D. T. Iskandar, G. Elbers, S. Sunaryono, S. Zubaidah, S. Sumari, Aulanni'am, A. B. Nandiyanto, I. Wibowo, & A. Y. Handaya (Eds.), International Conference on Life Science and Technology, ICoLiST 2019 Article 2607 (AIP Conference Proceedings; Vol. 2231). American Institute of Physics Inc.. https://doi.org/10.1063/5.0002607

Primaditya, Vanda ; Cory'ah, Fitra Arsy Nur ; Ariati, Linda Ika Puspita et al. / Effect of centella asiatica to the glucose transporter 4 and osteocalcin on the rotenonee-induced zebrafish larvae (Danio rerio) stunting model. International Conference on Life Science and Technology, ICoLiST 2019. editor / Ahmad Taufiq ; Hendra Susanto ; Hadi Nur ; Muhammad Aziz ; Chuang-Rung Chang ; Hyohyemi Lee ; Markus Diantoro ; Nandang Mufti ; Nik Ahmad Nizam Nik Malek ; I. Ching Wang ; Djoko Tjahnono Iskandar ; Gereon Elbers ; Sunaryono Sunaryono ; Siti Zubaidah ; Sumari Sumari ; Aulanni'am ; Asep Bayu Nandiyanto ; Indra Wibowo ; Adeodatus Yuda Handaya. American Institute of Physics Inc., 2020. (AIP Conference Proceedings).

@inproceedings{e48002daf8284e0bb33ab2ac3b560ef1,

title = "Effect of centella asiatica to the glucose transporter 4 and osteocalcin on the rotenonee-induced zebrafish larvae (Danio rerio) stunting model",

abstract = "Stunting is a failure of linear growth during the first two years of life. Rotenone is pesticide causes stunting. Rotenone as Endocrine Disrupting Chemicals and complex I mitochondrial inhibitor thus causes a decrease of ATP and oxidative stress. Centella asiatica is a plant that contains high antioxidants and antiinflammation. The purpose of this study is to evaluate the effect of CA ethanolic extract toward the rotenone-induced zebrafish larvae stunting model toward the expression increasing of GLUT 4 and Osteocalcin. This experimental study used zebrafish larvae as an animal model. Rotenone 12 ppb and CA extract were administered at 2-72 hpf. Observation of body length using optilab Image Raster at 3, 6, and 9 dpf, while the GLUT 4 and osteocalcin observed at 9 dpf by wholemount immunostaining. The results showed that rotenone 12.5 ppb decreased the body length of zebrafish larvae at 6 and 9 dpf significantly and more than 2 SD shorter from control. The expression of GLUT 4 and Osteocalcin in zebrafish larvae in the rotenone-induced group decreased significantly. Administration of 5 μg/mL extract increased expression of GLUT4, while the expression of osteocalcin increased by 2.5 μg/mL and 5 μg/mL. Rotenone 12.5 ppb induced stunting of zebrafish larvae. Ethanolic extract of CA protects the zebrafish larvae from stunting through increased expression of GLUT 4 and Osteocalcin.",

author = "Vanda Primaditya and Cory'ah, \{Fitra Arsy Nur\} and Ariati, \{Linda Ika Puspita\} and Zakiah and Wardani, \{Dyah Woro Kartiko Kusumo\} and Yuningsih and Dianita Primihastuti and Husnul Khotimah and Ali, \{Mohammad Muljohadi\} and Wibi Riawan",

note = "Publisher Copyright: {\textcopyright} 2020 American Institute of Physics Inc.. All rights reserved.; 2nd International Conference on Life Science and Technology, ICoLiST 2019 ; Conference date: 12-09-2019 Through 13-09-2019",

year = "2020",

month = apr,

day = "21",

doi = "10.1063/5.0002607",

language = "English",

series = "AIP Conference Proceedings",

publisher = "American Institute of Physics Inc.",

editor = "Ahmad Taufiq and Hendra Susanto and Hadi Nur and Muhammad Aziz and Chuang-Rung Chang and Hyohyemi Lee and Markus Diantoro and Nandang Mufti and Malek, \{Nik Ahmad Nizam Nik\} and Wang, \{I. Ching\} and Iskandar, \{Djoko Tjahnono\} and Gereon Elbers and Sunaryono Sunaryono and Siti Zubaidah and Sumari Sumari and Aulanni'am and Nandiyanto, \{Asep Bayu\} and Indra Wibowo and Handaya, \{Adeodatus Yuda\}",

booktitle = "International Conference on Life Science and Technology, ICoLiST 2019",

}

Primaditya, V, Cory'ah, FAN, Ariati, LIP, Zakiah, Wardani, DWKK, Yuningsih, Primihastuti, D, Khotimah, H, Ali, MM & Riawan, W 2020, Effect of centella asiatica to the glucose transporter 4 and osteocalcin on the rotenonee-induced zebrafish larvae (Danio rerio) stunting model. in A Taufiq, H Susanto, H Nur, M Aziz, C-R Chang, H Lee, M Diantoro, N Mufti, NANN Malek, IC Wang, DT Iskandar, G Elbers, S Sunaryono, S Zubaidah, S Sumari, Aulanni'am, AB Nandiyanto, I Wibowo & AY Handaya (eds), International Conference on Life Science and Technology, ICoLiST 2019., 2607, AIP Conference Proceedings, vol. 2231, American Institute of Physics Inc., 2nd International Conference on Life Science and Technology, ICoLiST 2019, Malang, East Java, Indonesia, 12/09/19. https://doi.org/10.1063/5.0002607

Effect of centella asiatica to the glucose transporter 4 and osteocalcin on the rotenonee-induced zebrafish larvae (Danio rerio) stunting model. / Primaditya, Vanda; Cory'ah, Fitra Arsy Nur; Ariati, Linda Ika Puspita et al.
International Conference on Life Science and Technology, ICoLiST 2019. ed. / Ahmad Taufiq; Hendra Susanto; Hadi Nur; Muhammad Aziz; Chuang-Rung Chang; Hyohyemi Lee; Markus Diantoro; Nandang Mufti; Nik Ahmad Nizam Nik Malek; I. Ching Wang; Djoko Tjahnono Iskandar; Gereon Elbers; Sunaryono Sunaryono; Siti Zubaidah; Sumari Sumari; Aulanni'am; Asep Bayu Nandiyanto; Indra Wibowo; Adeodatus Yuda Handaya. American Institute of Physics Inc., 2020. 2607 (AIP Conference Proceedings; Vol. 2231).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Effect of centella asiatica to the glucose transporter 4 and osteocalcin on the rotenonee-induced zebrafish larvae (Danio rerio) stunting model

AU - Primaditya, Vanda

AU - Cory'ah, Fitra Arsy Nur

AU - Ariati, Linda Ika Puspita

AU - Zakiah,

AU - Wardani, Dyah Woro Kartiko Kusumo

AU - Yuningsih,

AU - Primihastuti, Dianita

AU - Khotimah, Husnul

AU - Ali, Mohammad Muljohadi

AU - Riawan, Wibi

PY - 2020/4/21

Y1 - 2020/4/21

N2 - Stunting is a failure of linear growth during the first two years of life. Rotenone is pesticide causes stunting. Rotenone as Endocrine Disrupting Chemicals and complex I mitochondrial inhibitor thus causes a decrease of ATP and oxidative stress. Centella asiatica is a plant that contains high antioxidants and antiinflammation. The purpose of this study is to evaluate the effect of CA ethanolic extract toward the rotenone-induced zebrafish larvae stunting model toward the expression increasing of GLUT 4 and Osteocalcin. This experimental study used zebrafish larvae as an animal model. Rotenone 12 ppb and CA extract were administered at 2-72 hpf. Observation of body length using optilab Image Raster at 3, 6, and 9 dpf, while the GLUT 4 and osteocalcin observed at 9 dpf by wholemount immunostaining. The results showed that rotenone 12.5 ppb decreased the body length of zebrafish larvae at 6 and 9 dpf significantly and more than 2 SD shorter from control. The expression of GLUT 4 and Osteocalcin in zebrafish larvae in the rotenone-induced group decreased significantly. Administration of 5 μg/mL extract increased expression of GLUT4, while the expression of osteocalcin increased by 2.5 μg/mL and 5 μg/mL. Rotenone 12.5 ppb induced stunting of zebrafish larvae. Ethanolic extract of CA protects the zebrafish larvae from stunting through increased expression of GLUT 4 and Osteocalcin.

AB - Stunting is a failure of linear growth during the first two years of life. Rotenone is pesticide causes stunting. Rotenone as Endocrine Disrupting Chemicals and complex I mitochondrial inhibitor thus causes a decrease of ATP and oxidative stress. Centella asiatica is a plant that contains high antioxidants and antiinflammation. The purpose of this study is to evaluate the effect of CA ethanolic extract toward the rotenone-induced zebrafish larvae stunting model toward the expression increasing of GLUT 4 and Osteocalcin. This experimental study used zebrafish larvae as an animal model. Rotenone 12 ppb and CA extract were administered at 2-72 hpf. Observation of body length using optilab Image Raster at 3, 6, and 9 dpf, while the GLUT 4 and osteocalcin observed at 9 dpf by wholemount immunostaining. The results showed that rotenone 12.5 ppb decreased the body length of zebrafish larvae at 6 and 9 dpf significantly and more than 2 SD shorter from control. The expression of GLUT 4 and Osteocalcin in zebrafish larvae in the rotenone-induced group decreased significantly. Administration of 5 μg/mL extract increased expression of GLUT4, while the expression of osteocalcin increased by 2.5 μg/mL and 5 μg/mL. Rotenone 12.5 ppb induced stunting of zebrafish larvae. Ethanolic extract of CA protects the zebrafish larvae from stunting through increased expression of GLUT 4 and Osteocalcin.

UR - https://www.scopus.com/pages/publications/85092545957

U2 - 10.1063/5.0002607

DO - 10.1063/5.0002607

M3 - Conference contribution

AN - SCOPUS:85092545957

T3 - AIP Conference Proceedings

BT - International Conference on Life Science and Technology, ICoLiST 2019

A2 - Taufiq, Ahmad

A2 - Susanto, Hendra

A2 - Nur, Hadi

A2 - Aziz, Muhammad

A2 - Chang, Chuang-Rung

A2 - Lee, Hyohyemi

A2 - Diantoro, Markus

A2 - Mufti, Nandang

A2 - Malek, Nik Ahmad Nizam Nik

A2 - Wang, I. Ching

A2 - Iskandar, Djoko Tjahnono

A2 - Elbers, Gereon

A2 - Sunaryono, Sunaryono

A2 - Zubaidah, Siti

A2 - Sumari, Sumari

A2 - Aulanni'am, null

A2 - Nandiyanto, Asep Bayu

A2 - Wibowo, Indra

A2 - Handaya, Adeodatus Yuda

PB - American Institute of Physics Inc.

T2 - 2nd International Conference on Life Science and Technology, ICoLiST 2019

Y2 - 12 September 2019 through 13 September 2019

ER -

Primaditya V, Cory'ah FAN, Ariati LIP, Zakiah, Wardani DWKK, Yuningsih et al. Effect of centella asiatica to the glucose transporter 4 and osteocalcin on the rotenonee-induced zebrafish larvae (Danio rerio) stunting model. In Taufiq A, Susanto H, Nur H, Aziz M, Chang CR, Lee H, Diantoro M, Mufti N, Malek NANN, Wang IC, Iskandar DT, Elbers G, Sunaryono S, Zubaidah S, Sumari S, Aulanni'am, Nandiyanto AB, Wibowo I, Handaya AY, editors, International Conference on Life Science and Technology, ICoLiST 2019. American Institute of Physics Inc. 2020. 2607. (AIP Conference Proceedings). doi: 10.1063/5.0002607

Effect of centella asiatica to the glucose transporter 4 and osteocalcin on the rotenonee-induced zebrafish larvae (Danio rerio) stunting model

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