TY - GEN
T1 - Effect of Centella asiatica to developmental process of lead-induced zebrafish larvae
AU - Octaviana, Amrina
AU - Wari, Fitria Edni
AU - Noviasari, Damai
AU - Khotimah, Husnul
AU - Ali, M. Muljohadi
AU - Nudiana,
AU - Kalsum, Umi
N1 - Publisher Copyright:
© 2019 Author(s).
PY - 2019/6/4
Y1 - 2019/6/4
N2 - Lead (Pb) is one of the heavy metals which is harmful to the environment. Its toxicity due to the oxidative stress occur. Most lead studies focus on neurotoxic effects but little research directed at other tissues that affect the disruption of embryonic development. Centella asiatica (CA) has a nutrient content that acts as an antioxidant to balance oxidants in the cells so as to prevent oxidative stress. Besides C. asiatica can prevent disruption of embryonic development in the stage of proliferation and differentiation. The aim of this study is to evaluate the effect of CA extracts to prevent developmental disorders in Lead-induced zebrafish larvae. Zebrafish (D. rerio) embryos were exposed to Pb with concentration of 2,5 ppm and CA concentration of 1.25, 2.5, and 5 μg/mL. This study observed the survival rate, hatching rate, mortality rate, body length, heart rate, locomotor activity, and zebrafish larvae bone ossification during the development stage. The results showed that CA decreased mortality rate, increases hatching rate, and significantly decreases heart rate (p <0.05), increases body length, bone ossification (p <0.05) and motility in Pb-induced zebrafish larvae. So that it can be concluded that Centella asiatica can prevent developmental disorders in zebrafish larvae exposed to Pb.
AB - Lead (Pb) is one of the heavy metals which is harmful to the environment. Its toxicity due to the oxidative stress occur. Most lead studies focus on neurotoxic effects but little research directed at other tissues that affect the disruption of embryonic development. Centella asiatica (CA) has a nutrient content that acts as an antioxidant to balance oxidants in the cells so as to prevent oxidative stress. Besides C. asiatica can prevent disruption of embryonic development in the stage of proliferation and differentiation. The aim of this study is to evaluate the effect of CA extracts to prevent developmental disorders in Lead-induced zebrafish larvae. Zebrafish (D. rerio) embryos were exposed to Pb with concentration of 2,5 ppm and CA concentration of 1.25, 2.5, and 5 μg/mL. This study observed the survival rate, hatching rate, mortality rate, body length, heart rate, locomotor activity, and zebrafish larvae bone ossification during the development stage. The results showed that CA decreased mortality rate, increases hatching rate, and significantly decreases heart rate (p <0.05), increases body length, bone ossification (p <0.05) and motility in Pb-induced zebrafish larvae. So that it can be concluded that Centella asiatica can prevent developmental disorders in zebrafish larvae exposed to Pb.
KW - and Development Zebrafish Larvae
KW - Centella asiatica
KW - Lead
UR - https://www.scopus.com/pages/publications/85067021773
U2 - 10.1063/1.5110008
DO - 10.1063/1.5110008
M3 - Conference contribution
AN - SCOPUS:85067021773
T3 - AIP Conference Proceedings
BT - International Conference on Bioinformatics and Nanomedicine from Natural Resources for Biomedical Research
A2 - Budianto, Windy Yuliana
A2 - Sujuti, Hidayat
A2 - Khotimah, Husnul
PB - American Institute of Physics Inc.
T2 - 3rd Annual Scientific Meeting of Indonesian Consortium for Biomedical Sciences, ASMICBS 2018
Y2 - 21 November 2018 through 23 November 2018
ER -