Determination of solvent and ratio sample-solvent towards the production of oligo-glucosamine obtained from fermented tiger shrimp (Penaeus monodon) shell’s chitin by using precipitation method

Chitin obtained from the fermentation of Tiger Shrimp (Penaeus monodon) shell by using Providencia stuartii could be hydrolyzed into N-acetylglucosamine and another simple compound such as oligo-glucosamine. There is a limited research study regarding the isolation of oligo-glucosamine which also have a functional property. The study aimed to determine the best polar solvent and ratio sample and solvent (1:1 and 1:2, v/v) to precipitate oligo-glucosamine with the highest concentration. The polar solvents used in this study were ethanol, acetone, acetonitrile, ethanol-acetone (1:1, v/v), ethanolacetonitrile (1:1, v/v), and acetone-acetonitrile (1:1, v/v). Moreover, the yield obtained from precipitation by using those solvents were also investigated in this study. The result showed that combination of ethanol and acetonitrile (1:1, v/v) with ratio solvent-sample 1:1 (v/v) gave the highest concentration of oligo-glucosamine. The yield obtained was 0.12% (based on the volume obtained after fermentation) or 0.09% (based on the weight of chitin used for fermentation).