Decreased expression of MUC2 due to a decrease in the expression of lectins and apoptotic defects in colitis patients

Agung A. Wibowo; Bambang Pardjianto; Sutiman B. Sumitro; Nia Kania; Kusworini Handono

doi:10.1016/j.bbrep.2019.100655

Decreased expression of MUC2 due to a decrease in the expression of lectins and apoptotic defects in colitis patients

Agung A. Wibowo^*
, Bambang Pardjianto
, Sutiman B. Sumitro
, Nia Kania
, Kusworini Handono

^*Corresponding author for this work

Faculty of Medicine

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Introduction: The involvement of mucin, lectin, and apoptosis in colitis is still unclear. This study aimed to investigate changes in MUC2 expression, inflammation, and changes in lectin expression in colitis patients. Methods: A total of 17 patients were divided into two groups including 11 hemorrhoid patients as a control group and 6 colitis patients. MUC2 mutation analysis was carried out using immunofluorescent and FISH techniques. Assessment of caspase-3, Ki-67, NF-kB, and lectin expressions was also carried out by immunofluorescent technique then analyzed by confocal laser scanning microscope. Results: The MUC2, caspase-3, and lectin expressions were significantly lower in the colitis group than in the control group (p < 0.05). Conclusions: It was concluded that in colitis there was a change in MUC2 expression due to changes in lectins accompanied by apoptotic defects.conclusion

Original language	English
Article number	100655
Journal	Biochemistry and Biophysics Reports
Volume	19
DOIs	https://doi.org/10.1016/j.bbrep.2019.100655
Publication status	Published - Sept 2019

Keywords

Apoptosis
Colon
Inflammation
Lectin
Mucin

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10.1016/j.bbrep.2019.100655

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title = "Decreased expression of MUC2 due to a decrease in the expression of lectins and apoptotic defects in colitis patients",

abstract = "Introduction: The involvement of mucin, lectin, and apoptosis in colitis is still unclear. This study aimed to investigate changes in MUC2 expression, inflammation, and changes in lectin expression in colitis patients. Methods: A total of 17 patients were divided into two groups including 11 hemorrhoid patients as a control group and 6 colitis patients. MUC2 mutation analysis was carried out using immunofluorescent and FISH techniques. Assessment of caspase-3, Ki-67, NF-kB, and lectin expressions was also carried out by immunofluorescent technique then analyzed by confocal laser scanning microscope. Results: The MUC2, caspase-3, and lectin expressions were significantly lower in the colitis group than in the control group (p < 0.05). Conclusions: It was concluded that in colitis there was a change in MUC2 expression due to changes in lectins accompanied by apoptotic defects.conclusion",

keywords = "Apoptosis, Colon, Inflammation, Lectin, Mucin",

author = "Wibowo, \{Agung A.\} and Bambang Pardjianto and Sumitro, \{Sutiman B.\} and Nia Kania and Kusworini Handono",

note = "Publisher Copyright: {\textcopyright} 2019 The Authors",

year = "2019",

month = sep,

doi = "10.1016/j.bbrep.2019.100655",

language = "English",

volume = "19",

journal = "Biochemistry and Biophysics Reports",

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TY - JOUR

T1 - Decreased expression of MUC2 due to a decrease in the expression of lectins and apoptotic defects in colitis patients

AU - Wibowo, Agung A.

AU - Pardjianto, Bambang

AU - Sumitro, Sutiman B.

AU - Kania, Nia

AU - Handono, Kusworini

PY - 2019/9

Y1 - 2019/9

N2 - Introduction: The involvement of mucin, lectin, and apoptosis in colitis is still unclear. This study aimed to investigate changes in MUC2 expression, inflammation, and changes in lectin expression in colitis patients. Methods: A total of 17 patients were divided into two groups including 11 hemorrhoid patients as a control group and 6 colitis patients. MUC2 mutation analysis was carried out using immunofluorescent and FISH techniques. Assessment of caspase-3, Ki-67, NF-kB, and lectin expressions was also carried out by immunofluorescent technique then analyzed by confocal laser scanning microscope. Results: The MUC2, caspase-3, and lectin expressions were significantly lower in the colitis group than in the control group (p < 0.05). Conclusions: It was concluded that in colitis there was a change in MUC2 expression due to changes in lectins accompanied by apoptotic defects.conclusion

AB - Introduction: The involvement of mucin, lectin, and apoptosis in colitis is still unclear. This study aimed to investigate changes in MUC2 expression, inflammation, and changes in lectin expression in colitis patients. Methods: A total of 17 patients were divided into two groups including 11 hemorrhoid patients as a control group and 6 colitis patients. MUC2 mutation analysis was carried out using immunofluorescent and FISH techniques. Assessment of caspase-3, Ki-67, NF-kB, and lectin expressions was also carried out by immunofluorescent technique then analyzed by confocal laser scanning microscope. Results: The MUC2, caspase-3, and lectin expressions were significantly lower in the colitis group than in the control group (p < 0.05). Conclusions: It was concluded that in colitis there was a change in MUC2 expression due to changes in lectins accompanied by apoptotic defects.conclusion

KW - Apoptosis

KW - Colon

KW - Inflammation

KW - Lectin

KW - Mucin

UR - https://www.scopus.com/pages/publications/85066615063

U2 - 10.1016/j.bbrep.2019.100655

DO - 10.1016/j.bbrep.2019.100655

M3 - Article

AN - SCOPUS:85066615063

SN - 2405-5808

VL - 19

JO - Biochemistry and Biophysics Reports

JF - Biochemistry and Biophysics Reports

M1 - 100655

ER -

Decreased expression of MUC2 due to a decrease in the expression of lectins and apoptotic defects in colitis patients

Abstract

Keywords

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