Cytoplasm and nuclear crude protein proportion observed in pheripheral blood mono nuclear cells under senescence inducing stress exposure

Premature cellular senescence can be induced by exposing H2O2 in consentration-dependent manner. There is protein that contribute to changes driven early alteration in intracellular dynamic. Crude protein proportion in nuclear and cytoplasm were evaluated in this study for capturing acute stress response after 48 hours H202 exposure. Viability experiment were performed in isolated human PMBNCs which seeded in 6 plate well. After percentage of the surface of a culture dish covered by adherent cells >60%, cell was treated with 200 μm and 400 μm H202 for 48 hours incubate in 37 C. Harvesting cell with tripsin EDTA and nanodrop were performed to visualization crude protein proportion in nuclear and cytoplasm. Results and discussion, mean the cytoplasm crude protein were found to increased significanly in H202-treated cell (7.2 mg/ml + SD 0.1 in 200 μM and 7.6 mg/ml + SD 0.6 in 400 μM) than non-treated cell (6.5 mg/ml + SD 0.8) . H202 treatment led to dose-dependent increase cytoplasm crude protein but not in nuclear proportion rate. These results demonstrate that hydrogen peroxide triggers cellular senescence program with up regulated major cytoplasm crude protein. Elevation nuclear crude protein may contribute to another information related cellular response.