Cuttlefish (Sepia sp.) ink extract as antibacterial activity against aeromonas hydrophila

Aeromonas hydrophila is a gram negative opportunist bacterium associated with aquatic animal disease. Cephalopod ink has shown potential antiretroviral activity. The ink extracts of cuttlefish showed antibacterial effect. This study aims to investigate the antibacterial activity of the methanolic extract of the ink of cuttlefish (Sepia sp.) against Aeromonas hydrophilla. The shadedried ink sample from approximately 30g ink sacs obtained from 15 animals were immersed separately in methanol (1:3 w/v) solvents for overnight. Dried extract was used for the experiments. Isolate of Aeromonas hydrophila was originated from Jepara Brackishwater Aquaculture Center. The average yield percentage of cuttlefish tintan extract obtained was 4.86%. The results of the MIC test in table 5. show that the highest average absorbance value was obtained at a concentration of 50 ppm which was equal to 1,716 nm and the lowest absorbance was obtained at a treatment dose of 300 ppm at 0.841 nm while the Mc Farland tube was 0.933 nm. The results of antibacterial test on table 2 showed antibacterial activity of cuttlefish ink extract at concentration negative control showed diameter zone of 5 ± 1.2 mm, at positive control showed diameter zone of 31 ± 1.2 mm, at 250 ppm result 19 ± 0.9 mm, at 300 ppm result 22 ± 1.4 mm, at 350 ppm result 31 ± 1.2 mm.