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Community structure of H2S-oxidizing bacteria at Midorikawa mudflat, Japan

  • Irfan Mustafa*
  • , Ryuta Kawauchi
  • , Shigeru Morimura
  • *Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

This study aimed to determine the composition of H2S-oxidizing bacteria from Midorikawa mudflat using a culture-independent approach based on the sulfide quinone reductase (sqr) gene. This gene is involved in the initial step of H2S oxidation resulting in elemental sulfur. Total DNA was extracted from the mudflat sediment. A partial sequence of sqr was amplified by PCR and a clone library was established. The results showed that the community of H2S oxidizers was dominated by Alphaproteobacteria and Gammaproteobacteria. Sequence similarity of sqr to the isolated reference strains from GenBank database were 76-88%. However, a significant number of the clones were affiliated with uncultured bacteria. This indicated that diverse bacteria exist in Midorikawa mudflat to oxidize the released H2S, and most of them were previously undiscovered.

Original languageEnglish
Title of host publication9th International Conference on Global Resource Conservation, ICGRC and AJI from Ritsumeikan University
EditorsFahrul Zaman Bin Huyop, Dian Siswanto, Retno Mastuti, Chairat Treesubsuntorn
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417373
DOIs
Publication statusPublished - 10 Oct 2018
Event9th International Conference on Global Resource Conservation and AJI from Ritsumeikan University, ICGRC 2018 - Malang City - East Java, Indonesia
Duration: 7 Mar 20188 Mar 2018

Publication series

NameAIP Conference Proceedings
Volume2019
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference9th International Conference on Global Resource Conservation and AJI from Ritsumeikan University, ICGRC 2018
Country/TerritoryIndonesia
CityMalang City - East Java
Period7/03/188/03/18

Keywords

  • community structure
  • composition
  • HS-oxidizing bacteria
  • Midorikawa mudflat

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