BMP-15 m-RNA expression of mouse oocytes in vitro maturation in different droplet medium volume

Sri Rahayu; Nashi Widodo; Yumi Hoshino; Eimei Sato

doi:10.1016/j.apjr.2015.07.002

BMP-15 m-RNA expression of mouse oocytes in vitro maturation in different droplet medium volume

Sri Rahayu^*
, Nashi Widodo
, Yumi Hoshino
, Eimei Sato

^*Corresponding author for this work

Faculty of Mathematics and Natural Sciences

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Objective: To investigate droplet medium volume effect on the BMP-15 m-RNA expression. Methods: Oocytes are collected from mice ovaries by puncturing with a sterile 26-G needle. The droplet medium volumes are using 50 μL, 100 μL and 200 μL. The BMP-15 m-RNA expression is determined in each group. Results: The results indicated that BMP-15 m-RNA expression did not significantly difference when oocyte were cultured in 50 and 100 μL/droplet medium volume, but significantly difference (P < 0.05) when oocytes were cultured in 200 μL/droplet medium volume. Conclusions: The highest BMP-15 m-RNA expression occurs when oocytes are cultured in 200 μL/droplet medium volume.

Original language	English
Article number	17
Pages (from-to)	258-261
Number of pages	4
Journal	Asian Pacific Journal of Reproduction
Volume	4
Issue number	4
DOIs	https://doi.org/10.1016/j.apjr.2015.07.002
Publication status	Published - 1 Dec 2015

Keywords

BMP-15 m-RNA
Droplet medium volume
In vitro maturation
Mouse
Oocytes

Access to Document

10.1016/j.apjr.2015.07.002

Cite this

@article{ea8ff84996eb477497976837645165e5,

title = "BMP-15 m-RNA expression of mouse oocytes in vitro maturation in different droplet medium volume",

abstract = "Objective: To investigate droplet medium volume effect on the BMP-15 m-RNA expression. Methods: Oocytes are collected from mice ovaries by puncturing with a sterile 26-G needle. The droplet medium volumes are using 50 μL, 100 μL and 200 μL. The BMP-15 m-RNA expression is determined in each group. Results: The results indicated that BMP-15 m-RNA expression did not significantly difference when oocyte were cultured in 50 and 100 μL/droplet medium volume, but significantly difference (P < 0.05) when oocytes were cultured in 200 μL/droplet medium volume. Conclusions: The highest BMP-15 m-RNA expression occurs when oocytes are cultured in 200 μL/droplet medium volume.",

keywords = "BMP-15 m-RNA, Droplet medium volume, In vitro maturation, Mouse, Oocytes",

author = "Sri Rahayu and Nashi Widodo and Yumi Hoshino and Eimei Sato",

note = "Publisher Copyright: {\textcopyright} 2015 Hainan Medical College.",

year = "2015",

month = dec,

day = "1",

doi = "10.1016/j.apjr.2015.07.002",

language = "English",

volume = "4",

pages = "258--261",

journal = "Asian Pacific Journal of Reproduction",

issn = "2305-0500",

publisher = "Wolters Kluwer Medknow Publications",

number = "4",

}

TY - JOUR

T1 - BMP-15 m-RNA expression of mouse oocytes in vitro maturation in different droplet medium volume

AU - Rahayu, Sri

AU - Widodo, Nashi

AU - Hoshino, Yumi

AU - Sato, Eimei

PY - 2015/12/1

Y1 - 2015/12/1

N2 - Objective: To investigate droplet medium volume effect on the BMP-15 m-RNA expression. Methods: Oocytes are collected from mice ovaries by puncturing with a sterile 26-G needle. The droplet medium volumes are using 50 μL, 100 μL and 200 μL. The BMP-15 m-RNA expression is determined in each group. Results: The results indicated that BMP-15 m-RNA expression did not significantly difference when oocyte were cultured in 50 and 100 μL/droplet medium volume, but significantly difference (P < 0.05) when oocytes were cultured in 200 μL/droplet medium volume. Conclusions: The highest BMP-15 m-RNA expression occurs when oocytes are cultured in 200 μL/droplet medium volume.

AB - Objective: To investigate droplet medium volume effect on the BMP-15 m-RNA expression. Methods: Oocytes are collected from mice ovaries by puncturing with a sterile 26-G needle. The droplet medium volumes are using 50 μL, 100 μL and 200 μL. The BMP-15 m-RNA expression is determined in each group. Results: The results indicated that BMP-15 m-RNA expression did not significantly difference when oocyte were cultured in 50 and 100 μL/droplet medium volume, but significantly difference (P < 0.05) when oocytes were cultured in 200 μL/droplet medium volume. Conclusions: The highest BMP-15 m-RNA expression occurs when oocytes are cultured in 200 μL/droplet medium volume.

KW - BMP-15 m-RNA

KW - Droplet medium volume

KW - In vitro maturation

KW - Mouse

KW - Oocytes

UR - https://www.scopus.com/pages/publications/85006201182

U2 - 10.1016/j.apjr.2015.07.002

DO - 10.1016/j.apjr.2015.07.002

M3 - Article

AN - SCOPUS:85006201182

SN - 2305-0500

VL - 4

SP - 258

EP - 261

JO - Asian Pacific Journal of Reproduction

JF - Asian Pacific Journal of Reproduction

IS - 4

M1 - 17

ER -

BMP-15 m-RNA expression of mouse oocytes in vitro maturation in different droplet medium volume

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this