Biocellulose isolated from the waste of pinecone flower (Pinus merkusii Jungh et de Vriese)

A bio-cellulose isolated from pinecone flower waste has been isolated by two type procedures. The first method is commonly used in industrial protocol. It is undergone via delignification and bleaching processes. In the delignification process provide a black-liquid of lignin waste, meanwhile the bleaching process afford neutralized clear liquid. The bio-cellulose is the main product. On the hand, the second method is initiated by soxhlet extraction using three different solvents i.e. n-hexane, ethanol and toluene, respectively. The extraction process produces organic hexane extract, ethanol extract, and toluene extract. All these extract have different appearance in color and prospective. Further delignification and bleaching process provide black lignin waste and neutralized liquid waste. The bio-cellulose still also become a major product.