Bioactivity of Moringa oleifera and Albumin Formulation in Controlling TNF-α and lFN-γ Production by NK Cells in Mice Model Type 1 Diabetes

Type 1 diabetes (T1D) is a disease caused by pancreatic beta-cell injury due to high pro-inflammatory cytokines. TNF-α and IFN-γ are pro-inflammatory cytokines that play a role in T1D progression. Moringa oleifera (MO) and albumin (A) have anti-inflammatory and antidiabetic effects. The combination of both can works synergistically in suppressing the production of pro-inflammatory molecules. This study was conducted using five different groups (healthy mice, T1D, D1, D2, and D3). A dose of 145 mg/kg BW streptozotocin was used to induce T1D in mice. Moringa oleifera and albumin formulation (MOA) were orally administered for 14 days. Dose 1 (800 mg/kg MO:800 mg/kg A), dose 2 (615 mg/kg MO:615 mg/kg A), and dose 3 (800 mg/kg MO:615 mg/kg A). On day 15, hepatic cells from mice were isolated post-treatment, and the profile of NK⁺TNF-α⁺ and NK⁺IFN-γ⁺ were analyzed by flow cytometry. This study reports that MOA administered in D3 more effectively suppresses TNF-α and IFN-γ produced by NK cells. MOA could be synergies work to decrease or suppress the level TNF-α and IFN-γ in T1D. So, administered MOA had the potential to be used as an alternative medicine for DM.