TY - GEN
T1 - Bacteriocin production of Lactobacillus sp. from intestines of ducks (Anas domesticus L.) incubated at room temperature and antibacterial effectivity against pathogen
AU - Arfani, Nurfitri
AU - Nur, Fatmawati
AU - Hafsan,
AU - Azrianingsih, Rodiyati
N1 - Publisher Copyright:
© 2017 Author(s).
PY - 2017/5/17
Y1 - 2017/5/17
N2 - Bacteriocin is a peptide that is easily degraded by proteolytic enzymes in the digestive systems of animals, including humans. It has antimicrobial activity against pathogenic bacteria. Lactobacillus sp. is one type of lactic acid bacteria (LAB) that occupies the intestines of ducks (Anas domesticus L.). The purpose of this research was to determine the optimum time of the highest protein production by Lactobacillus sp. and to determine inhibitory activity of bacteriocin against pathogenic bacteria (Escherichia coli and Staphylococcus aureus). Using the Bradford method, the results showed that the optimum time of highest bacteriocin production was after 36 hours of incubation, with a protein content of 0.93 mg/ml. The bacteriocin inhibitory activity against Escherichia coli showed that a protein concentration of 30% gave a maximum inhibition index of 1.1 mm, while for Staphylococcus aureus, a concentration of 70% gave a maximum inhibition index of 0.3 mm. Further research is required to determine the stationary state of bacteriocin production in this circumstance.
AB - Bacteriocin is a peptide that is easily degraded by proteolytic enzymes in the digestive systems of animals, including humans. It has antimicrobial activity against pathogenic bacteria. Lactobacillus sp. is one type of lactic acid bacteria (LAB) that occupies the intestines of ducks (Anas domesticus L.). The purpose of this research was to determine the optimum time of the highest protein production by Lactobacillus sp. and to determine inhibitory activity of bacteriocin against pathogenic bacteria (Escherichia coli and Staphylococcus aureus). Using the Bradford method, the results showed that the optimum time of highest bacteriocin production was after 36 hours of incubation, with a protein content of 0.93 mg/ml. The bacteriocin inhibitory activity against Escherichia coli showed that a protein concentration of 30% gave a maximum inhibition index of 1.1 mm, while for Staphylococcus aureus, a concentration of 70% gave a maximum inhibition index of 0.3 mm. Further research is required to determine the stationary state of bacteriocin production in this circumstance.
UR - https://www.scopus.com/pages/publications/85020189185
U2 - 10.1063/1.4983431
DO - 10.1063/1.4983431
M3 - Conference contribution
AN - SCOPUS:85020189185
T3 - AIP Conference Proceedings
BT - 7th International Conference on Global Resource Conservation
A2 - Mustafa, Irfan
A2 - Shrestha, Bhupal Govinda
A2 - Hamid, Nazefah Abdul
PB - American Institute of Physics Inc.
T2 - 7th International Conference on Global Resource Conservation: From Traditional Herbal Medicine into Synthetic Biology for Better Human Lives, ICGRC 2016
Y2 - 2 November 2016 through 4 November 2016
ER -