Authentication of patin (pangasius micronemus) fish oil adulterated with palm oil using ftir spectroscopy combined with chemometrics

Objective: The goal of this research was to perform authentication of patin [Pangasius micronemus) fish oil (PFO) adulterated with palm oil (PO) using FTIR spectroscopy combined with chemometrics method. Methods: Patin fish oil (PFO) and PFO adulterated with palm oil (PO) were measured using FTIR instrument at wavenumbers region of 4000-650 cm1. The chemometrics methods, namely multivariate calibration of partial least square (PLS) and principal component regression (PCR) were used to make calibration and validation models during quantification. Discriminant analysis (DA) was used to make grouping pure PFO and PFO adulterated with PO. Results: The results showed that PLS and PCR could be used to quantify PO as adulterant in PFO, either in calibration or validation models. FTIR spectroscopy combined with multivariate calibration offered accurate and precise method for quantitative analysis with R² value of >0.999 and low RMSEC and RMSEP. DA was capable of grouping PFO and PFO adulterated with PO with an accuracy level of 100%. Conclusion: FTIR spectroscopy combined with chemometrics could be reliable technique for quantification and discrimination of PFO and PFO adulterated with PO.