Antibacterial Effect of Coffea canephora Ethanolic Extract through Potassium and Magnesium Efflux Mechanism

Ibnu Diptya Prawatya; Daniel Winatakusuma; Ferdian Tanaka; Dwi Yuni Nur Hidayati; Hidayat Sujuti

doi:10.1063/5.0118070

Antibacterial Effect of Coffea canephora Ethanolic Extract through Potassium and Magnesium Efflux Mechanism

Ibnu Diptya Prawatya
, Daniel Winatakusuma
, Ferdian Tanaka
, Dwi Yuni Nur Hidayati^*
, Hidayat Sujuti

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

The resistance of some bacteria towards current antibiotics calls for a new development of antibiotics. Robusta coffee (Coffea canephora) contains active compounds with antibacterial effects such as chlorogenic acid, caffeine, caffeic acid, and quinic acid. Intracellular ions such as potassium and magnesium have important roles in bacterial life. This study aims to investigate the potential antibacterial effect of Coffea canephora and its possibility in damaging bacterial cell walls and membranes through efflux of potassium and magnesium ions. This experimental study used three types of bacteria: Methicillin-resistant Staphylococcus aureus (MRSA), Klebsiella pneumoniae, and Pseudomonas aeruginosa, each divided into a control group and an experimental group with different concentrations. Efflux of potassium and magnesium ions in bacteria was measured using an atomic absorption spectrophotometer (AAS), while changes in bacterial cell wall morphology were observed using a scanning electron microscope (SEM). Coffea canephora is found to significantly increase the magnesium efflux of all bacteria compared to the control group (p<0.05), while the potassium efflux only significantly increases in MRSA and Klebsiella pneumoniae. In observations using SEM, changes in the morphology of bacterial cell walls are found in the experimental group, indicating a damaging effect caused by Coffea canephora.

Original language	English
Title of host publication	4th International Conference on Life Science and Technology, ICoLiST 2023
Editors	Ahmad Taufiq, Hendra Susanto, I. Ching Wang, Hadi Nur, Muhammad Aziz, Chuang-Rung Chang, Gereon Elbers, Sri Rahayu Lestari, Markus Diantoro, Nandang Mufti, Chen Siang Ng, Nik Ahmad Nizam Nik Malek, Sunaryono, Yu-Hsin Chiu, Siti Zubaidah, Aulanni'am, Indra Wibowo, Adeodatus Yuda Handaya
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735443136
DOIs	https://doi.org/10.1063/5.0118070
Publication status	Published - 24 Jan 2023
Externally published	Yes
Event	4th International Conference on Life Science and Technology, ICoLiST 2023 - Virtual, Online, Indonesia Duration: 31 Aug 2021 → …

Publication series

Name	AIP Conference Proceedings
Volume	2634
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	4th International Conference on Life Science and Technology, ICoLiST 2023
Country/Territory	Indonesia
City	Virtual, Online
Period	31/08/21 → …

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Access to Document

10.1063/5.0118070

Cite this

Prawatya, I. D., Winatakusuma, D., Tanaka, F., Hidayati, D. Y. N., & Sujuti, H. (2023). Antibacterial Effect of Coffea canephora Ethanolic Extract through Potassium and Magnesium Efflux Mechanism. In A. Taufiq, H. Susanto, I. C. Wang, H. Nur, M. Aziz, C.-R. Chang, G. Elbers, S. R. Lestari, M. Diantoro, N. Mufti, C. S. Ng, N. A. N. N. Malek, Sunaryono, Y.-H. Chiu, S. Zubaidah, Aulanni'am, I. Wibowo, & A. Y. Handaya (Eds.), 4th International Conference on Life Science and Technology, ICoLiST 2023 Article 020088 (AIP Conference Proceedings; Vol. 2634). American Institute of Physics Inc.. https://doi.org/10.1063/5.0118070

Prawatya, Ibnu Diptya ; Winatakusuma, Daniel ; Tanaka, Ferdian et al. / Antibacterial Effect of Coffea canephora Ethanolic Extract through Potassium and Magnesium Efflux Mechanism. 4th International Conference on Life Science and Technology, ICoLiST 2023. editor / Ahmad Taufiq ; Hendra Susanto ; I. Ching Wang ; Hadi Nur ; Muhammad Aziz ; Chuang-Rung Chang ; Gereon Elbers ; Sri Rahayu Lestari ; Markus Diantoro ; Nandang Mufti ; Chen Siang Ng ; Nik Ahmad Nizam Nik Malek ; Sunaryono ; Yu-Hsin Chiu ; Siti Zubaidah ; Aulanni'am ; Indra Wibowo ; Adeodatus Yuda Handaya. American Institute of Physics Inc., 2023. (AIP Conference Proceedings).

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title = "Antibacterial Effect of Coffea canephora Ethanolic Extract through Potassium and Magnesium Efflux Mechanism",

abstract = "The resistance of some bacteria towards current antibiotics calls for a new development of antibiotics. Robusta coffee (Coffea canephora) contains active compounds with antibacterial effects such as chlorogenic acid, caffeine, caffeic acid, and quinic acid. Intracellular ions such as potassium and magnesium have important roles in bacterial life. This study aims to investigate the potential antibacterial effect of Coffea canephora and its possibility in damaging bacterial cell walls and membranes through efflux of potassium and magnesium ions. This experimental study used three types of bacteria: Methicillin-resistant Staphylococcus aureus (MRSA), Klebsiella pneumoniae, and Pseudomonas aeruginosa, each divided into a control group and an experimental group with different concentrations. Efflux of potassium and magnesium ions in bacteria was measured using an atomic absorption spectrophotometer (AAS), while changes in bacterial cell wall morphology were observed using a scanning electron microscope (SEM). Coffea canephora is found to significantly increase the magnesium efflux of all bacteria compared to the control group (p<0.05), while the potassium efflux only significantly increases in MRSA and Klebsiella pneumoniae. In observations using SEM, changes in the morphology of bacterial cell walls are found in the experimental group, indicating a damaging effect caused by Coffea canephora.",

author = "Prawatya, \{Ibnu Diptya\} and Daniel Winatakusuma and Ferdian Tanaka and Hidayati, \{Dwi Yuni Nur\} and Hidayat Sujuti",

note = "Publisher Copyright: {\textcopyright} 2023 American Institute of Physics Inc.. All rights reserved.; 4th International Conference on Life Science and Technology, ICoLiST 2023 ; Conference date: 31-08-2021",

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month = jan,

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doi = "10.1063/5.0118070",

language = "English",

series = "AIP Conference Proceedings",

publisher = "American Institute of Physics Inc.",

editor = "Ahmad Taufiq and Hendra Susanto and Wang, \{I. Ching\} and Hadi Nur and Muhammad Aziz and Chuang-Rung Chang and Gereon Elbers and Lestari, \{Sri Rahayu\} and Markus Diantoro and Nandang Mufti and Ng, \{Chen Siang\} and Malek, \{Nik Ahmad Nizam Nik\} and Sunaryono and Yu-Hsin Chiu and Siti Zubaidah and Aulanni'am and Indra Wibowo and Handaya, \{Adeodatus Yuda\}",

booktitle = "4th International Conference on Life Science and Technology, ICoLiST 2023",

}

Prawatya, ID, Winatakusuma, D, Tanaka, F, Hidayati, DYN & Sujuti, H 2023, Antibacterial Effect of Coffea canephora Ethanolic Extract through Potassium and Magnesium Efflux Mechanism. in A Taufiq, H Susanto, IC Wang, H Nur, M Aziz, C-R Chang, G Elbers, SR Lestari, M Diantoro, N Mufti, CS Ng, NANN Malek, Sunaryono, Y-H Chiu, S Zubaidah, Aulanni'am, I Wibowo & AY Handaya (eds), 4th International Conference on Life Science and Technology, ICoLiST 2023., 020088, AIP Conference Proceedings, vol. 2634, American Institute of Physics Inc., 4th International Conference on Life Science and Technology, ICoLiST 2023, Virtual, Online, Indonesia, 31/08/21. https://doi.org/10.1063/5.0118070

Antibacterial Effect of Coffea canephora Ethanolic Extract through Potassium and Magnesium Efflux Mechanism. / Prawatya, Ibnu Diptya; Winatakusuma, Daniel; Tanaka, Ferdian et al.
4th International Conference on Life Science and Technology, ICoLiST 2023. ed. / Ahmad Taufiq; Hendra Susanto; I. Ching Wang; Hadi Nur; Muhammad Aziz; Chuang-Rung Chang; Gereon Elbers; Sri Rahayu Lestari; Markus Diantoro; Nandang Mufti; Chen Siang Ng; Nik Ahmad Nizam Nik Malek; Sunaryono; Yu-Hsin Chiu; Siti Zubaidah; Aulanni'am; Indra Wibowo; Adeodatus Yuda Handaya. American Institute of Physics Inc., 2023. 020088 (AIP Conference Proceedings; Vol. 2634).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Antibacterial Effect of Coffea canephora Ethanolic Extract through Potassium and Magnesium Efflux Mechanism

AU - Prawatya, Ibnu Diptya

AU - Winatakusuma, Daniel

AU - Tanaka, Ferdian

AU - Hidayati, Dwi Yuni Nur

AU - Sujuti, Hidayat

PY - 2023/1/24

Y1 - 2023/1/24

N2 - The resistance of some bacteria towards current antibiotics calls for a new development of antibiotics. Robusta coffee (Coffea canephora) contains active compounds with antibacterial effects such as chlorogenic acid, caffeine, caffeic acid, and quinic acid. Intracellular ions such as potassium and magnesium have important roles in bacterial life. This study aims to investigate the potential antibacterial effect of Coffea canephora and its possibility in damaging bacterial cell walls and membranes through efflux of potassium and magnesium ions. This experimental study used three types of bacteria: Methicillin-resistant Staphylococcus aureus (MRSA), Klebsiella pneumoniae, and Pseudomonas aeruginosa, each divided into a control group and an experimental group with different concentrations. Efflux of potassium and magnesium ions in bacteria was measured using an atomic absorption spectrophotometer (AAS), while changes in bacterial cell wall morphology were observed using a scanning electron microscope (SEM). Coffea canephora is found to significantly increase the magnesium efflux of all bacteria compared to the control group (p<0.05), while the potassium efflux only significantly increases in MRSA and Klebsiella pneumoniae. In observations using SEM, changes in the morphology of bacterial cell walls are found in the experimental group, indicating a damaging effect caused by Coffea canephora.

AB - The resistance of some bacteria towards current antibiotics calls for a new development of antibiotics. Robusta coffee (Coffea canephora) contains active compounds with antibacterial effects such as chlorogenic acid, caffeine, caffeic acid, and quinic acid. Intracellular ions such as potassium and magnesium have important roles in bacterial life. This study aims to investigate the potential antibacterial effect of Coffea canephora and its possibility in damaging bacterial cell walls and membranes through efflux of potassium and magnesium ions. This experimental study used three types of bacteria: Methicillin-resistant Staphylococcus aureus (MRSA), Klebsiella pneumoniae, and Pseudomonas aeruginosa, each divided into a control group and an experimental group with different concentrations. Efflux of potassium and magnesium ions in bacteria was measured using an atomic absorption spectrophotometer (AAS), while changes in bacterial cell wall morphology were observed using a scanning electron microscope (SEM). Coffea canephora is found to significantly increase the magnesium efflux of all bacteria compared to the control group (p<0.05), while the potassium efflux only significantly increases in MRSA and Klebsiella pneumoniae. In observations using SEM, changes in the morphology of bacterial cell walls are found in the experimental group, indicating a damaging effect caused by Coffea canephora.

UR - https://www.scopus.com/pages/publications/85147301457

U2 - 10.1063/5.0118070

DO - 10.1063/5.0118070

M3 - Conference contribution

AN - SCOPUS:85147301457

T3 - AIP Conference Proceedings

BT - 4th International Conference on Life Science and Technology, ICoLiST 2023

A2 - Taufiq, Ahmad

A2 - Susanto, Hendra

A2 - Wang, I. Ching

A2 - Nur, Hadi

A2 - Aziz, Muhammad

A2 - Chang, Chuang-Rung

A2 - Elbers, Gereon

A2 - Lestari, Sri Rahayu

A2 - Diantoro, Markus

A2 - Mufti, Nandang

A2 - Ng, Chen Siang

A2 - Malek, Nik Ahmad Nizam Nik

A2 - Sunaryono, null

A2 - Chiu, Yu-Hsin

A2 - Zubaidah, Siti

A2 - Aulanni'am, null

A2 - Wibowo, Indra

A2 - Handaya, Adeodatus Yuda

PB - American Institute of Physics Inc.

T2 - 4th International Conference on Life Science and Technology, ICoLiST 2023

Y2 - 31 August 2021

ER -

Prawatya ID, Winatakusuma D, Tanaka F, Hidayati DYN, Sujuti H. Antibacterial Effect of Coffea canephora Ethanolic Extract through Potassium and Magnesium Efflux Mechanism. In Taufiq A, Susanto H, Wang IC, Nur H, Aziz M, Chang CR, Elbers G, Lestari SR, Diantoro M, Mufti N, Ng CS, Malek NANN, Sunaryono, Chiu YH, Zubaidah S, Aulanni'am, Wibowo I, Handaya AY, editors, 4th International Conference on Life Science and Technology, ICoLiST 2023. American Institute of Physics Inc. 2023. 020088. (AIP Conference Proceedings). doi: 10.1063/5.0118070

Antibacterial Effect of Coffea canephora Ethanolic Extract through Potassium and Magnesium Efflux Mechanism

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