Anti-leukemic activity of Cyperus rotundus L. on human acute myeloid leukemia HL-60 cells in vitro

Sulistyo Mulyo Agustini; Edi Widjajanto; Muhaimin Rifa’i; Sofia Mubarika Haryana; Nurdiana; Diana Lyrawati; Usi Sukorini; Noviana Dwi Lestari

doi:10.56499/jppres22.1502_11.1.191

Anti-leukemic activity of Cyperus rotundus L. on human acute myeloid leukemia HL-60 cells in vitro

Sulistyo Mulyo Agustini
, Edi Widjajanto
, Muhaimin Rifa’i
, Sofia Mubarika Haryana
, Nurdiana
, Diana Lyrawati
, Usi Sukorini
, Noviana Dwi Lestari^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Context: Acute myeloid leukemia (AML) is the most common form of acute leukemia. Currently, many people use medicinal herbs for the treatment of cancers. Nut grass (Cyperus rotundus L.) is a medicinal plant widely used in conventional medicine due to its role as an anti-cancer. Aims: To evaluate the effect of Cyperus rotundus tuber (CRT) ethanolic extract on cell proliferation, differentiation, cell cycle, and apoptotic of HL-60 cells. Methods: HL-60 cells line as a model for AML was cultured under the influence of CRT extract concentrations (35.4, 354, and 3540 µg/mL) for 48 h. Furthermore, the cell was subjected to carboxyfluorescein succinimidyl ester staining for proliferation, using a CD117 marker for differentiation. Annexin V-FITC and PI for cell cycle and apoptosis. The effect of this herb was studied by flow cytometry. The data were statistically analyzed with one-way ANOVA (p≤0.05) and the Tukey test using SPSS version 16 for Windows. Results: The results showed that the CRT inhibited proliferation activity, differentiation, cell cycle arrest, and apoptosis. Conclusions: The data clearly showed the potential anti-cancer activity of CRT on HL-60 cells and suggested that it could help develop promising therapeutic agents for AML treatments.

Original language	English
Pages (from-to)	191-197
Number of pages	7
Journal	Journal of Pharmacy and Pharmacognosy Research
Volume	11
Issue number	1
DOIs	https://doi.org/10.56499/jppres22.1502_11.1.191
Publication status	Published - Jan 2023

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

acute myeloid leukemia
apoptosis
cell cycle
Cyperus rotundus
HL-60 cells line

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10.56499/jppres22.1502_11.1.191

Cite this

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title = "Anti-leukemic activity of Cyperus rotundus L. on human acute myeloid leukemia HL-60 cells in vitro",

abstract = "Context: Acute myeloid leukemia (AML) is the most common form of acute leukemia. Currently, many people use medicinal herbs for the treatment of cancers. Nut grass (Cyperus rotundus L.) is a medicinal plant widely used in conventional medicine due to its role as an anti-cancer. Aims: To evaluate the effect of Cyperus rotundus tuber (CRT) ethanolic extract on cell proliferation, differentiation, cell cycle, and apoptotic of HL-60 cells. Methods: HL-60 cells line as a model for AML was cultured under the influence of CRT extract concentrations (35.4, 354, and 3540 µg/mL) for 48 h. Furthermore, the cell was subjected to carboxyfluorescein succinimidyl ester staining for proliferation, using a CD117 marker for differentiation. Annexin V-FITC and PI for cell cycle and apoptosis. The effect of this herb was studied by flow cytometry. The data were statistically analyzed with one-way ANOVA (p≤0.05) and the Tukey test using SPSS version 16 for Windows. Results: The results showed that the CRT inhibited proliferation activity, differentiation, cell cycle arrest, and apoptosis. Conclusions: The data clearly showed the potential anti-cancer activity of CRT on HL-60 cells and suggested that it could help develop promising therapeutic agents for AML treatments.",

keywords = "acute myeloid leukemia, apoptosis, cell cycle, Cyperus rotundus, HL-60 cells line",

author = "Agustini, \{Sulistyo Mulyo\} and Edi Widjajanto and Muhaimin Rifa{\textquoteright}i and Haryana, \{Sofia Mubarika\} and Nurdiana and Diana Lyrawati and Usi Sukorini and Lestari, \{Noviana Dwi\}",

note = "Publisher Copyright: {\textcopyright} 2023 Journal of Pharmacy \& Pharmacognosy Research.",

year = "2023",

month = jan,

doi = "10.56499/jppres22.1502\_11.1.191",

language = "English",

volume = "11",

pages = "191--197",

journal = "Journal of Pharmacy and Pharmacognosy Research",

issn = "0719-4250",

publisher = "Academic Association of Pharmaceutical Sciences from Antofagasta (ASOCIFA)",

number = "1",

}

TY - JOUR

T1 - Anti-leukemic activity of Cyperus rotundus L. on human acute myeloid leukemia HL-60 cells in vitro

AU - Agustini, Sulistyo Mulyo

AU - Widjajanto, Edi

AU - Rifa’i, Muhaimin

AU - Haryana, Sofia Mubarika

AU - Nurdiana,

AU - Lyrawati, Diana

AU - Sukorini, Usi

AU - Lestari, Noviana Dwi

PY - 2023/1

Y1 - 2023/1

N2 - Context: Acute myeloid leukemia (AML) is the most common form of acute leukemia. Currently, many people use medicinal herbs for the treatment of cancers. Nut grass (Cyperus rotundus L.) is a medicinal plant widely used in conventional medicine due to its role as an anti-cancer. Aims: To evaluate the effect of Cyperus rotundus tuber (CRT) ethanolic extract on cell proliferation, differentiation, cell cycle, and apoptotic of HL-60 cells. Methods: HL-60 cells line as a model for AML was cultured under the influence of CRT extract concentrations (35.4, 354, and 3540 µg/mL) for 48 h. Furthermore, the cell was subjected to carboxyfluorescein succinimidyl ester staining for proliferation, using a CD117 marker for differentiation. Annexin V-FITC and PI for cell cycle and apoptosis. The effect of this herb was studied by flow cytometry. The data were statistically analyzed with one-way ANOVA (p≤0.05) and the Tukey test using SPSS version 16 for Windows. Results: The results showed that the CRT inhibited proliferation activity, differentiation, cell cycle arrest, and apoptosis. Conclusions: The data clearly showed the potential anti-cancer activity of CRT on HL-60 cells and suggested that it could help develop promising therapeutic agents for AML treatments.

AB - Context: Acute myeloid leukemia (AML) is the most common form of acute leukemia. Currently, many people use medicinal herbs for the treatment of cancers. Nut grass (Cyperus rotundus L.) is a medicinal plant widely used in conventional medicine due to its role as an anti-cancer. Aims: To evaluate the effect of Cyperus rotundus tuber (CRT) ethanolic extract on cell proliferation, differentiation, cell cycle, and apoptotic of HL-60 cells. Methods: HL-60 cells line as a model for AML was cultured under the influence of CRT extract concentrations (35.4, 354, and 3540 µg/mL) for 48 h. Furthermore, the cell was subjected to carboxyfluorescein succinimidyl ester staining for proliferation, using a CD117 marker for differentiation. Annexin V-FITC and PI for cell cycle and apoptosis. The effect of this herb was studied by flow cytometry. The data were statistically analyzed with one-way ANOVA (p≤0.05) and the Tukey test using SPSS version 16 for Windows. Results: The results showed that the CRT inhibited proliferation activity, differentiation, cell cycle arrest, and apoptosis. Conclusions: The data clearly showed the potential anti-cancer activity of CRT on HL-60 cells and suggested that it could help develop promising therapeutic agents for AML treatments.

KW - acute myeloid leukemia

KW - apoptosis

KW - cell cycle

KW - Cyperus rotundus

KW - HL-60 cells line

UR - https://www.scopus.com/pages/publications/85149932174

U2 - 10.56499/jppres22.1502_11.1.191

DO - 10.56499/jppres22.1502_11.1.191

M3 - Article

AN - SCOPUS:85149932174

SN - 0719-4250

VL - 11

SP - 191

EP - 197

JO - Journal of Pharmacy and Pharmacognosy Research

JF - Journal of Pharmacy and Pharmacognosy Research

IS - 1

ER -

Anti-leukemic activity of Cyperus rotundus L. on human acute myeloid leukemia HL-60 cells in vitro

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