TY - GEN
T1 - Anti-inflammatory effects of Centella asiatica ethanolic extracts towards indomethacin-induced gastric ulcer model in rats by altering COX-2 expression
AU - Thirza, Savannah Quila
AU - Pratiwi, Made Dinda
AU - Noviardi, Dwi Evan Prima Putra
AU - Sriepinndonta, Priscilla Maiselina
AU - Fitriani, Fatimah Nur
AU - Kalsum, Umi
AU - Khotimah, Husnul
AU - Mintaroem, Karyono
AU - Norahmawati, Eviana
N1 - Publisher Copyright:
© 2021 Author(s).
PY - 2021/5/25
Y1 - 2021/5/25
N2 - Non-streoidal anti-inflammatory drugs (NSAIDs) are commonly used despite their known gastrointestinal adverse effects. Centella asiatica (CA) is widely used for its anti-inflammatory due to its active components. This study was conducted to investigate the anti-inflammatory effects of CA ethanolic (CAE) extracts in gastric ulcer by measuring COX-2 expression. The effects of CAE were identified in mice (Rattus norvegicus) used as peptic ulcer models induced with Indometachin at 30 mg/kg BW. The experimental groups were divided into five groups: negative control group, positive control group day 8, and the treatment groups receiving three different doses of CAE (100, 200, and 400 mg/kg BW). CAE was administered once daily for seven days. The experimental models were sacrificed 24 hours after the last CAE administration, and COX-2 expression in the gastric mucosal layer was assessed using immunohistochemistry. Hematoxylin-eosin (HE) staining was used to examine gastric mucosal damage. There was a consistent significant decrease in COX-2 expression in all treatment groups compared to control groups (p = 0.006). The results showed that CAE displayed anti-inflammatory effect. CAE extracts could decrease COX-2 expression in all three treatment groups compared to control models. CAE has a potential to be anti-inflammatory herbal therapy for gastric ulcers.
AB - Non-streoidal anti-inflammatory drugs (NSAIDs) are commonly used despite their known gastrointestinal adverse effects. Centella asiatica (CA) is widely used for its anti-inflammatory due to its active components. This study was conducted to investigate the anti-inflammatory effects of CA ethanolic (CAE) extracts in gastric ulcer by measuring COX-2 expression. The effects of CAE were identified in mice (Rattus norvegicus) used as peptic ulcer models induced with Indometachin at 30 mg/kg BW. The experimental groups were divided into five groups: negative control group, positive control group day 8, and the treatment groups receiving three different doses of CAE (100, 200, and 400 mg/kg BW). CAE was administered once daily for seven days. The experimental models were sacrificed 24 hours after the last CAE administration, and COX-2 expression in the gastric mucosal layer was assessed using immunohistochemistry. Hematoxylin-eosin (HE) staining was used to examine gastric mucosal damage. There was a consistent significant decrease in COX-2 expression in all treatment groups compared to control groups (p = 0.006). The results showed that CAE displayed anti-inflammatory effect. CAE extracts could decrease COX-2 expression in all three treatment groups compared to control models. CAE has a potential to be anti-inflammatory herbal therapy for gastric ulcers.
UR - https://www.scopus.com/pages/publications/85107291113
U2 - 10.1063/5.0053025
DO - 10.1063/5.0053025
M3 - Conference contribution
AN - SCOPUS:85107291113
T3 - AIP Conference Proceedings
BT - International Conference on Life Sciences and Technology, ICoLiST 2020
A2 - Taufiq, Ahmad
A2 - Susanto, Hendra
A2 - Nur, Hadi
A2 - Aziz, Muhammad
A2 - Suksuwan, Acharee
A2 - Ng, Chen Siang
A2 - Jemon, Khairunadwa Binti
A2 - Amin, Mohamad
A2 - Diantoro, Markus
A2 - Mufti, Nandang
A2 - Malek, Nik Ahmad Nizam Nik
A2 - Wang, I. Ching
A2 - Sunaryono, null
A2 - Zubaidah, Siti
A2 - Aulanni'am, null
A2 - Wibowo, Indra
A2 - Handaya, Adeodatus Yuda
PB - American Institute of Physics Inc.
T2 - 3rd International Conference on Life Sciences and Technology, ICoLiST 2020
Y2 - 29 September 2020
ER -