Anti-inflammatory effect of Black cincau (Mesona palustris BL) based supplement in male carrageenan-induced mice

This study aims to investigate the potency and optimum dose of Mesona palustris supplement as an anti-inflammatory in carrageenan-induced mice (Musmus culus). In this study, twenty male Balb/C mice were divided into five groups. The treatment groups are including a negative control, positive control, drug control, and two different doses of black cincau based herbal supplement (BCBHS) groups. In this experiment, edema was observed surrounding mice feet, and the relative number of NF-α and CD68 were measured using flow cytometry analysis. The results revealed that both of BCBHS doses were effective to inhibit edema incidence in carrageenan-induced mice. The expression level of TNF-α and CD68 in both of BCBHS doses were lower than the positive control. Further, the tissue recovery was observed with M. palustris supplementation. Our result suggests that both of BCBHS doses have an anti-inflammatory effect in carrageenan-induced mice.