Anti-inflammatory action of Indonesian Black Garlic (IBG) ethanol extracts in LPS-stimulated RAW 264.7 macrophage cells

Indonesian Black Garlic (IBG) ethanol extracts are a new type of fresh garlic product, produced at high a temperature and humidity. IBG has active antioxidant activity. This study aimed to assess the anti-inflammatory actions of IBG in RAW 264.7 macrophage cells. The cells were grown and treated with various concentrations of IBG extracts (50 to 1000mg/L). Cell counting kit-8 assay revealed that IBG was not toxic up to 400mg/L. IBG significantly reduced the expression of inflammation-mediated expression in LPS-stimulated RAW 264.7 macrophage cells. IBG was also found to suppress inflammation cytokines. This is evident in mRNA expression and reduced expression of iNOS, COX-2, IL-6, NF-kB, and TNF-α at protein levels. The results suggest that IBG can be used to prevent or treat diseases associated with inflammation.