TY - JOUR
T1 - Anti-aging evaluation of transfersome and nanostructured lipid carrier gels containing Curcuma longa and Glycyrrhiza glabra extract on ultraviolet-B-irradiated rats
AU - Adianingsih, Oktavia Rahayu
AU - Latifani, Salma
AU - Fitriani, Annisa Nur
AU - Wardani, Anistasya Dwitama
AU - Qurrotuain, Iffah
AU - Wahyudi, Rachmad Tri
AU - Dewi, Azizah Maharani Putri
AU - Dhinata, Jessica Aurellia Wahyu
AU - Rumabutar, Gloria Angelica
AU - Puspita, Oktavia Eka
AU - Ihsan, Bacthiar Rifai Pratita
AU - Riawan, Wibi
AU - Danimayostu, Adeltrudis Adelsa
AU - Yurina, Valentina
N1 - Publisher Copyright:
Copyright Adianingsih OR et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) https://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2025
Y1 - 2025
N2 - Skin aging is a multifactorial process influenced by intrinsic and extrinsic factors, particularly UV-B radiation. Natural ingredients such as Curcuma longa and Glycyrrhiza glabra show potential anti-aging effects. Transfersomes and nanostructured lipid carriers (NLCs) are delivery systems that have the potential to be developed for anti-aging applications. This study aimed to formulate transfersome and NLC systems loaded with turmeric and licorice extracts (TFS-CGE and NLC-CGE), evaluate their anti-aging effects through in vitro studies, and assess anti-photoaging effects in gel dosage form through an in vivo study. TFS-CGE and NLC-CGE formulations were developed using thin-layer hydration and hot homogenization methods, respectively, with optimization of lipid–surfactant and solid–liquid lipid ratios. The resulting nanoparticles were characterized for particle size, polydispersity index, zeta potential, morphology, entrapment efficiency, drug loading, and deformability. In vitro assays confirmed that both formulations exhibited antioxidant and anti-collagenase activities. Gel formulations of the optimized TFS-CGE and NLC-CGE were prepared for in vivo application. A UV-B-induced photoaging model in Wistar rats was used to evaluate anti-aging efficacy. After 3 weeks of topical treatment, TFS-CGE and NLC-CGE gels significantly improved skin elasticity, reduced epidermal thickness, and enhanced collagen density. Immunohistochemical analysis showed increased expression of elastin, TGF-β, and collagen types I, II, and III, along with reduced MMP-1 expression, indicating protective effects against UV-B-induced skin aging. These findings demonstrate that TFS-CGE and NLC-CGE gels are effective anti-aging formulations and help enhance skin delivery and bioactivity through a nanotechnology-based approach.
AB - Skin aging is a multifactorial process influenced by intrinsic and extrinsic factors, particularly UV-B radiation. Natural ingredients such as Curcuma longa and Glycyrrhiza glabra show potential anti-aging effects. Transfersomes and nanostructured lipid carriers (NLCs) are delivery systems that have the potential to be developed for anti-aging applications. This study aimed to formulate transfersome and NLC systems loaded with turmeric and licorice extracts (TFS-CGE and NLC-CGE), evaluate their anti-aging effects through in vitro studies, and assess anti-photoaging effects in gel dosage form through an in vivo study. TFS-CGE and NLC-CGE formulations were developed using thin-layer hydration and hot homogenization methods, respectively, with optimization of lipid–surfactant and solid–liquid lipid ratios. The resulting nanoparticles were characterized for particle size, polydispersity index, zeta potential, morphology, entrapment efficiency, drug loading, and deformability. In vitro assays confirmed that both formulations exhibited antioxidant and anti-collagenase activities. Gel formulations of the optimized TFS-CGE and NLC-CGE were prepared for in vivo application. A UV-B-induced photoaging model in Wistar rats was used to evaluate anti-aging efficacy. After 3 weeks of topical treatment, TFS-CGE and NLC-CGE gels significantly improved skin elasticity, reduced epidermal thickness, and enhanced collagen density. Immunohistochemical analysis showed increased expression of elastin, TGF-β, and collagen types I, II, and III, along with reduced MMP-1 expression, indicating protective effects against UV-B-induced skin aging. These findings demonstrate that TFS-CGE and NLC-CGE gels are effective anti-aging formulations and help enhance skin delivery and bioactivity through a nanotechnology-based approach.
KW - aging
KW - nanoparticle
KW - NLC
KW - transfersome
KW - UV-B
UR - https://www.scopus.com/pages/publications/105030982285
U2 - 10.3897/pharmacia.72.e174188
DO - 10.3897/pharmacia.72.e174188
M3 - Article
AN - SCOPUS:105030982285
SN - 0428-0296
JO - Pharmacia
JF - Pharmacia
IS - 72
ER -